| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 97,990 | A→C | T247P (ACC→CCC) | murC → | UDP‑N‑acetylmuramate‑‑L‑alanine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 97,990 | 0 | A | C | 95.8% | 82.6 / ‑3.0 | 24 | T247P (ACC→CCC) | murC | UDP‑N‑acetylmuramate‑‑L‑alanine ligase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (10/13); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCC‑GTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCGCCA > NZ_CP009273/97856‑98118 | cctaccAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCC‑GTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCa < 1:336875/138‑1 (MQ=255)cctaccAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCC‑GTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCa < 1:462258/138‑1 (MQ=255) gggCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCC‑GTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTAc > 1:462099/1‑139 (MQ=255) tttAAAACAGACTTTTATTAATTTTCTGCACAACCTGCC‑GTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGa < 1:312838/135‑1 (MQ=255) tttAAAACAGACTTTTATTAATTTTCTGCACAACCTGCC‑GTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGa > 2:312838/1‑135 (MQ=255) aCTTTTATTAATTTTCTGCACAACCTGCC‑GTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGAcgtgc > 2:70951/1‑139 (MQ=255) cTTTTATTAATTTTCTGCACAACCTGCCGGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGAcgtgc < 2:159624/139‑1 (MQ=255) ttaATTTTCTGCACAACCTGCC‑GTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTaga < 1:70951/139‑1 (MQ=255) tttCTGCACAACCTGCC‑GTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGAt > 1:39024/1‑138 (MQ=255) tACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGAcgtgc < 2:45370/106‑1 (MQ=255) tACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGAcgtgc > 1:45370/1‑106 (MQ=255) tACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAg < 2:39024/138‑1 (MQ=255) cGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAggg < 2:522217/138‑1 (MQ=255) atCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACa > 1:461747/1‑138 (MQ=255) atCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACa > 1:531660/1‑138 (MQ=255) gTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGc < 2:461747/139‑1 (MQ=255) gTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGc < 1:166381/139‑1 (MQ=255) cgcgAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGAt < 2:71475/137‑1 (MQ=255) cgcgAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGAt > 1:71475/1‑137 (MQ=255) cgAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATgcgc > 1:49340/1‑139 (MQ=255) aaTTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGt < 1:275452/139‑1 (MQ=255) gTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATgcgc < 2:562224/139‑1 (MQ=255) tGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATgcgc > 2:164591/1‑138 (MQ=255) gggCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCGCCa < 2:531660/138‑1 (MQ=255) | CCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCC‑GTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCGCCA > NZ_CP009273/97856‑98118 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |