Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,418,201	A→G	L171P (CTG→CCG)	hisM ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,418,201	0	A	G	100.0%	54.0 / NA	17	L171P (CTG→CCG)	hisM	ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/7);  total (10/7)

ATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAAC  >  NZ_CP009273/2418075‑2418336                                                                                                                               |                                                                                                                                        atgatTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCACTAGAGTGCGGCATCAGGATCa                                                                                                                             >  2:382516/1‑139 (MQ=255)        aaTAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGtt                                                                                                                      >  1:386397/1‑139 (MQ=255)         aTAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTg                                                                                                                     <  1:514598/139‑1 (MQ=255)              gCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTa                                                                                                                >  2:264460/1‑139 (MQ=255)                      gCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTa                                                                                                        <  1:264460/139‑1 (MQ=255)                                                aTACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCaa                                                                               >  2:137562/1‑138 (MQ=255)                                                          gcgTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGc                                                                    >  1:238674/1‑139 (MQ=255)                                                                     gcgGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACAtttt                                                          >  1:483377/1‑138 (MQ=255)                                                                               ttCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCgag                                                <  1:137562/138‑1 (MQ=255)                                                                                       aTCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCAt                                         <  2:508133/137‑1 (MQ=255)                                                                                       aTCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCAt                                         >  1:508133/1‑137 (MQ=255)                                                                                                  tGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTa                                                                                                        <  2:238078/63‑1 (MQ=255)                                                                                                  tGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTa                                                                                                        >  1:238078/1‑63 (MQ=255)                                                                                                  tGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTggcggc                            >  1:265009/1‑139 (MQ=255)                                                                                                  tGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTGCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAAGAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTggcggc                            <  2:258968/139‑1 (MQ=255)                                                                                                            tGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTcc                  <  2:483377/139‑1 (MQ=255)                                                                                                                            tGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAAc  >  2:108928/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                        ATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAAC  >  NZ_CP009273/2418075‑2418336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑