Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,786,008 T→G G459G (GGT→GGG)  gabD → NADP‑dependent succinate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,786,0080TG100.0% 89.4 / NA 25G459G (GGT→GGG) gabDNADP‑dependent succinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/16);  total (9/16)

GCCGCCTATTTCTACGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTCAGGCGATT  >  NZ_CP009273/2785874‑2786138
                                                                                                                                      |                                                                                                                                  
gccgccTATTTCTACGCCCGTAATTTAAGCCGTGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTg                                                                                                                                 <  1:424595/138‑1 (MQ=255)
              cGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGtt                                                                                                                    <  1:429493/137‑1 (MQ=255)
                    tggtttAAGGCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGCTCGGCGGCATCAAAGCCTCGGGGCTGTGTCGTGAAGGTTCGAAGTAt                                                                                                            >  2:160730/4‑139 (MQ=255)
                                tgTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCg                                                                                                                  >  1:375011/2‑121 (MQ=255)
                                tgTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCg                                                                                                                  <  2:375011/120‑1 (MQ=255)
                                               cGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCaaa                                                                                 <  2:83799/139‑1 (MQ=255)
                                               cGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCaaa                                                                                 <  2:250918/139‑1 (MQ=255)
                                               cGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCaaa                                                                                 <  1:533636/139‑1 (MQ=255)
                                                        ggAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATAtgtg                                                                         >  1:510002/1‑138 (MQ=255)
                                                          aGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                      <  2:510002/139‑1 (MQ=255)
                                                          aGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                      <  2:143883/139‑1 (MQ=255)
                                                            tACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCg                                                                    >  2:535423/1‑139 (MQ=255)
                                                            tACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCg                                                                    >  1:271991/1‑139 (MQ=255)
                                                              cGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCg                                                                                                                  <  1:461757/91‑1 (MQ=255)
                                                              cGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCg                                                                                                                  >  2:461757/1‑91 (MQ=255)
                                                                 cATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCaaa                                                                                 >  1:283740/1‑121 (MQ=255)
                                                                 cATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCaaa                                                                                 <  2:283740/121‑1 (MQ=255)
                                                                                  ccGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCAGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGa                                               <  1:411274/138‑1 (MQ=255)
                                                                                      cATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGaa                                          <  1:568487/139‑1 (MQ=255)
                                                                                                         gCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGa                                                                                                    <  2:195068/62‑1 (MQ=255)
                                                                                                         gCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGa                                                                                                    >  1:195068/1‑62 (MQ=255)
                                                                                                                  ggcggcATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGcgccg               <  2:77479/138‑1 (MQ=255)
                                                                                                                       cATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATg                                                                                                           <  2:206275/41‑1 (MQ=255)
                                                                                                                       cATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATg                                                                                                           >  1:206275/1‑41 (MQ=255)
                                                                                                                              gCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTCAGGCGAtt  <  1:231239/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
GCCGCCTATTTCTACGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTCAGGCGATT  >  NZ_CP009273/2785874‑2786138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑