Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,926,085	A→C	G96G (GGT→GGG)	fucO ←	lactaldehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,926,085	0	A	C	100.0%	169.4 / NA	47	G96G (GGT→GGG)	fucO	lactaldehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/26);  total (21/26)

GCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCACCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGCATCCATCTTATC  >  NZ_CP009273/2925951‑2926219                                                                                                                                       |                                                                                                                                       gctgTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAacccccac                                                                                                                                    >  1:437371/1‑139 (MQ=255)      ggTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCaa                                                                                                                                 >  1:28263/1‑137 (MQ=255)      ggTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCaa                                                                                                                                 >  1:4577/1‑137 (MQ=255)          ggAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCg                                                                                                                            <  2:4577/138‑1 (MQ=255)          ggAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGa                                                                                                                           >  2:330325/1‑139 (MQ=255)               tGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAgg                                                                                                                      >  1:359877/1‑139 (MQ=255)                       tCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCTCCAATAGCGATCAGGTAATCcgc                                                                                                              <  1:423638/139‑1 (MQ=255)                           tACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          >  1:146071/1‑139 (MQ=255)                           tACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          <  1:237233/139‑1 (MQ=255)                           tACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          <  1:321418/139‑1 (MQ=255)                                tGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          <  1:357511/134‑1 (MQ=255)                                tGGGCTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACACCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          >  2:357511/1‑134 (MQ=255)                                 gggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTc                                                                                                    >  2:120726/1‑139 (MQ=255)                                  ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTAtt                                                                                                     <  2:359877/137‑1 (MQ=255)                                  ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTAtt                                                                                                     <  2:146071/137‑1 (MQ=255)                                      tATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGa                                                                                                <  2:121493/138‑1 (MQ=255)                                        ttGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTa                                                                                                       <  1:400378/129‑1 (MQ=255)                                        ttGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTa                                                                                                       >  2:400378/1‑129 (MQ=255)                                                          tCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTa                                                                                                       <  2:29144/111‑1 (MQ=255)                                                          tCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTa                                                                                                       >  1:29144/1‑111 (MQ=255)                                                          tCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGaa                                                                                               >  1:14125/1‑119 (MQ=255)                                                          tCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGaa                                                                                               <  2:14125/119‑1 (MQ=255)                                                            cAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTgac                                                                          <  2:449926/138‑1 (MQ=255)                                                                 tACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          >  2:225313/1‑101 (MQ=255)                                                                 tACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          <  1:225313/101‑1 (MQ=255)                                                                   cGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGt                                                                    <  2:28263/137‑1 (MQ=255)                                                                   cGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTaa                                                                  >  2:136021/1‑139 (MQ=255)                                                                   cGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTaa                                                                  <  1:187367/139‑1 (MQ=255)                                                                         tCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAAttgttg                                                            >  1:550574/1‑139 (MQ=255)                                                                          cGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          <  1:392990/92‑1 (MQ=255)                                                                          cGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          >  2:392990/1‑92 (MQ=255)                                                                             cAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTgttgggtt                                                        <  1:239723/139‑1 (MQ=255)                                                                             cAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTgttgggtt                                                        <  1:428652/139‑1 (MQ=255)                                                                             cAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTgttgggtt                                                        <  2:313094/139‑1 (MQ=255)                                                                                           ttgCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCcgt                                          >  2:186133/1‑139 (MQ=255)                                                                                            tgCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCcgtc                                         >  1:484234/1‑139 (MQ=255)                                                                                                 aTAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTaaa                                    <  1:330325/139‑1 (MQ=255)                                                                                                       ccAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGcc                               <  1:136021/138‑1 (MQ=255)                                                                                                           tCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGaa                                                                                               >  1:359484/1‑70 (MQ=255)                                                                                                           tCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGaa                                                                                               <  2:359484/70‑1 (MQ=255)                                                                                                           tCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTgacga                                                                        <  1:64824/93‑1 (MQ=255)                                                                                                           tCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTgacga                                                                        >  2:64824/1‑93 (MQ=255)                                                                                                           tCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGc                          <  2:324068/139‑1 (MQ=255)                                                                                                           tCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGc                          <  1:120726/139‑1 (MQ=255)                                                                                                                              tgGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGACCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGcatccatc       >  2:518789/1‑139 (MQ=255)                                                                                                                                   aaCCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACg                                              <  2:84365/95‑1 (MQ=255)                                                                                                                                   aaCCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACg                                              >  1:84365/1‑95 (MQ=255)                                                                                                                                   aaCCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGCATCCATCTTATc  >  2:126623/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       GCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCACCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGCATCCATCTTATC  >  NZ_CP009273/2925951‑2926219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑