| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,027,998 | T→C | E179G (GAA→GGA) | lysS ← | lysine‑‑tRNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,027,998 | 0 | T | C | 100.0% | 80.7 / NA | 23 | E179G (GAA→GGA) | lysS | lysine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/11); total (12/11) | |||||||||||
ATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGA > NZ_CP009273/3027863‑3028125 | aTAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCAt < 2:1382/139‑1 (MQ=255) catcatCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcg < 2:233628/138‑1 (MQ=255) cgGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTAt > 2:282511/1‑139 (MQ=255) aCTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACg > 2:97125/1‑139 (MQ=255) aCTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACg > 1:352101/1‑139 (MQ=255) gCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCa < 1:97125/137‑1 (MQ=255) cgcACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt < 2:127208/136‑1 (MQ=255) cgcACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt > 1:127208/1‑136 (MQ=255) cTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCa < 2:240369/108‑1 (MQ=255) cTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCa > 1:240369/1‑108 (MQ=255) tgtTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa < 2:118292/139‑1 (MQ=255) ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcg < 1:5078/50‑1 (MQ=255) ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcg > 2:5078/1‑50 (MQ=255) ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACg < 2:161914/95‑1 (MQ=255) ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACg > 1:161914/1‑95 (MQ=255) ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTgg > 1:307809/1‑106 (MQ=255) ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTgg < 2:307809/106‑1 (MQ=255) ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCg > 1:531556/1‑123 (MQ=255) ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCg < 2:531556/123‑1 (MQ=255) ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa > 1:203351/1‑137 (MQ=255) ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa > 1:138299/1‑137 (MQ=255) gCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCgg > 1:86754/1‑90 (MQ=255) gCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCgg < 2:86754/90‑1 (MQ=255) | ATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGA > NZ_CP009273/3027863‑3028125 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |