Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,104,251 T→G T79P (ACC→CCC)  yghD ← GspM family type II secretion system protein YghD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,104,2510TG100.0% 129.3 / NA 35T79P (ACC→CCC) yghDGspM family type II secretion system protein YghD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/20);  total (15/20)

TCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACG  >  NZ_CP009273/3104121‑3104383
                                                                                                                                  |                                                                                                                                    
tCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCg                                                                                                                              >  2:472144/1‑139 (MQ=255)
   gCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGcca                                                                                                                           >  1:28550/1‑139 (MQ=255)
         cGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAgc                                                                                                                      <  1:277809/138‑1 (MQ=255)
          gCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAgctg                                                                                                                    <  2:218530/139‑1 (MQ=255)
          gCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAgctg                                                                                                                    >  2:362861/1‑139 (MQ=255)
               atgaCCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGctgctg                                                                                                                 <  1:535340/137‑1 (MQ=255)
               atgaCCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCa                                                                                                               <  2:57455/139‑1 (MQ=255)
                    caccGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATAtt                                                                                                          >  2:112126/1‑139 (MQ=255)
                       cGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGtc                                                                                                       <  2:12051/139‑1 (MQ=255)
                         aGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGtctc                                                                                                     >  1:206834/1‑139 (MQ=255)
                         aGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGtctc                                                                                                     >  1:35350/1‑139 (MQ=255)
                             cgcgGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTg                                                                                                  <  2:186725/138‑1 (MQ=255)
                                          tCACTGGTGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGtt                                                                                    >  2:144732/1‑139 (MQ=255)
                                          tCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGtt                                                                                    >  2:384042/1‑139 (MQ=255)
                                          tCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGtt                                                                                    <  2:154732/139‑1 (MQ=255)
                                          tCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGtt                                                                                    <  2:35350/139‑1 (MQ=255)
                                          tCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGtt                                                                                    >  1:154732/1‑139 (MQ=255)
                                                atgaCCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTc                                                                               >  1:64517/1‑138 (MQ=255)
                                                  gaCCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAata                                                                                                            <  2:154497/107‑1 (MQ=255)
                                                  gaCCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAata                                                                                                            >  1:154497/1‑107 (MQ=255)
                                                   aCCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACg                                                                           <  1:472144/139‑1 (MQ=255)
                                                    cccGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAgc                                                                                                                      >  2:244189/1‑95 (MQ=255)
                                                    cccGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAgc                                                                                                                      <  1:244189/95‑1 (MQ=255)
                                                     ccGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGc                                                                         <  1:384042/139‑1 (MQ=255)
                                                      cgcgATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCt                                                                        <  1:91004/139‑1 (MQ=255)
                                                                    aGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACg                                                          <  2:28550/139‑1 (MQ=255)
                                                                                ggCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTg                                                                                               <  2:156395/90‑1 (MQ=255)
                                                                                ggCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTg                                                                                               >  1:156395/1‑90 (MQ=255)
                                                                                ggCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGc                                                                      <  2:125258/115‑1 (MQ=255)
                                                                                ggCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGc                                                                      >  1:125258/1‑115 (MQ=255)
                                                                                 gCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGc                                             <  2:79148/139‑1 (MQ=255)
                                                                                                   ataataTCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCg                           <  2:64517/139‑1 (MQ=255)
                                                                                                              cgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGc                                                                      <  2:120407/85‑1 (MQ=255)
                                                                                                              cgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGc                                                                      >  1:120407/1‑85 (MQ=255)
                                                                                                                            gTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAcg  <  1:144732/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                    
TCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACG  >  NZ_CP009273/3104121‑3104383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑