BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,111,922	T→C	N184D (AAT→GAT)	sslE ←	lipoprotein metalloprotease SslE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,111,922	0	T	C	100.0%	132.1 / NA	36	N184D (AAT→GAT)	sslE	lipoprotein metalloprotease SslE
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/17); total (19/17)

AGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGA  >  NZ_CP009273/3111791‑3112057

agcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGa                                                                                                                                  <  1:96338/139‑1 (MQ=255)

agcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGa                                                                                                                                  <  1:106586/139‑1 (MQ=255)

          tCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTa                                                                                                                         <  1:524814/138‑1 (MQ=255)

            cACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTg                                                                                                                      >  1:408199/1‑139 (MQ=255)

               ctctTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGAc                                                                                                                   >  1:511250/1‑139 (MQ=255)

                 ctTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCTGTCCCTCTTTTCCTGGACGt                                                                                                                 >  1:302999/1‑139 (MQ=255)

                          ccAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGa                                                                                                        <  1:216837/139‑1 (MQ=255)

                            aGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGaaa                                                                                                      >  1:470469/1‑139 (MQ=255)

                              cTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAAcc                                                                                                    >  2:566035/1‑139 (MQ=255)

                                 tttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCg                                                                                                   <  2:5473/137‑1 (MQ=255)

                                         ctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCgg                                                                                                                                   <  2:132163/97‑1 (MQ=255)

                                         ctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCgg                                                                                                                                   >  1:132163/1‑97 (MQ=255)

                                                    aGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCg                                                                               >  2:79925/1‑138 (MQ=255)

                                                    aGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCg                                                                               >  2:75847/1‑138 (MQ=255)

                                                          aTTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCg                                                                        >  2:164422/1‑139 (MQ=255)

                                                                       gCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAGCGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc                                                           <  1:245853/139‑1 (MQ=255)

                                                                       gCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTTTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc                                                           <  1:566035/139‑1 (MQ=255)

                                                                        cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCg                                                                                                                                       >  1:58811/1‑62 (MQ=255)

                                                                        cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCg                                                                                                                                       <  2:58811/62‑1 (MQ=255)

                                                                        cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGcgcg                                                          >  2:153095/1‑139 (MQ=255)

                                                                        cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGcgcg                                                          >  2:500845/1‑139 (MQ=255)

                                                                          aGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTc                                                        >  2:178893/1‑139 (MQ=255)

                                                                              gAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTc                                                     <  1:445385/138‑1 (MQ=255)

                                                                                 gggTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAgg                                                 <  2:543005/137‑1 (MQ=255)

                                                                                             gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGac                                        <  1:79925/136‑1 (MQ=255)

                                                                                              aTCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGac                                        <  2:161176/135‑1 (MQ=255)

                                                                                              aTCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGac                                        >  1:161176/1‑135 (MQ=255)

                                                                                                            cGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGCTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcgcacgc                      <  1:483700/139‑1 (MQ=255)

                                                                                                                 gACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGc                  >  1:120143/1‑138 (MQ=255)

                                                                                                                      aCCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcgcg            <  1:500845/139‑1 (MQ=255)

                                                                                                                      aCCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcgcg            <  1:197879/139‑1 (MQ=255)

                                                                                                                           ttCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCt       >  2:268266/1‑139 (MQ=255)

                                                                                                                                tATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctga  >  2:164582/1‑139 (MQ=255)

                                                                                                                                tATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctga  >  2:265477/1‑139 (MQ=255)

                                                                                                                                tATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctga  >  1:558210/1‑139 (MQ=255)

                                                                                                                                tATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTc     <  2:558210/136‑1 (MQ=255)

AGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGA  >  NZ_CP009273/3111791‑3112057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`