Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,778,563 (A)8→7 intergenic (‑193/+558) BW25113_RS25705 ← / ← BW25113_RS13825 DUF5507 domain‑containing protein/tRNA‑Ile

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,778,5560A.93.3% 51.7 / ‑1.2 15intergenic (‑186/+565)BW25113_RS25705/BW25113_RS13825DUF5507 domain‑containing protein/tRNA‑Ile
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (8/6);  total (8/7)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTGTCACTATAAAAAAACGCTCATTTGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGATAAAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTG  >  NZ_CP009273/2778420‑2778687
                                                                                                                                        |                                                                                                                                   
gTTTGTCACTATAAAAAAACGCTCATTTGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGATaa                                                                                                                                    >  1:763878/1‑138 (MQ=255)
   tGTCACTATAAAAAAACGCTCATTTGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGATaaaaa                                                                                                                                 <  2:501042/138‑1 (MQ=255)
   tGTCACTATAAAAAAACGCTCATTTGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGATaaaaa                                                                                                                                 >  2:194647/1‑138 (MQ=255)
                                            ttAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAgtgt                                                                                        >  2:701870/1‑137 (MQ=255)
                                             tAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAgtgt                                                                                        >  1:229794/1‑136 (MQ=255)
                                             tAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAgtgt                                                                                        >  1:539845/1‑136 (MQ=255)
                                                      cACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGATAAAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTta                                                                              <  2:763878/138‑1 (MQ=255)
                                                       aCTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTatat                                                                           <  1:430845/139‑1 (MQ=255)
                                                       aCTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTatat                                                                           <  2:229794/139‑1 (MQ=255)
                                                       aCTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTatat                                                                           <  1:194647/139‑1 (MQ=255)
                                                            cTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCaa                                                                      <  1:371311/139‑1 (MQ=255)
                                                                 cATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCg                                                                 <  1:60043/139‑1 (MQ=255)
                                                                                      aGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCa                                             <  2:336929/138‑1 (MQ=255)
                                                                                                       tttAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTca                            >  1:149412/1‑138 (MQ=255)
                                                                                                            taaACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAAc                      >  2:762056/1‑139 (MQ=255)
                                                                                                                 aaaaGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATc                 >  2:263704/1‑139 (MQ=255)
                                                                                                                          tttCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTgcgg        >  1:497283/1‑139 (MQ=255)
                                                                                                                                tGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGgtg  >  1:143166/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                   
GTTTGTCACTATAAAAAAACGCTCATTTGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGATAAAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTG  >  NZ_CP009273/2778420‑2778687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑