Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,974,914	Δ1 bp	coding (628/1419 nt)	araE ←	arabinose‑proton symporter AraE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,974,914	0	G	.	100.0%	265.8 / NA	60	coding (628/1419 nt)	araE	arabinose‑proton symporter AraE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (35/25);  total (35/25)

ATCTTAAACAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAA  >  NZ_CP009273/2974790‑2975047                                                                                                                             |                                                                                                                                      aTCTTAAACAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCgccag                                                                                                                        >  2:633770/1‑139 (MQ=255)      aaaCAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCcagccagc                                                                                                                   >  2:403690/1‑139 (MQ=255)            tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCtt                                                                                                                                 >  1:454130/1‑119 (MQ=255)            tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCtt                                                                                                                                 <  2:454130/119‑1 (MQ=255)            tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGcgcg                                                                                                                >  1:546452/1‑136 (MQ=255)            tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGcgcg                                                                                                                <  2:546452/136‑1 (MQ=255)            tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  2:712249/1‑139 (MQ=255)            tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  2:126386/1‑139 (MQ=255)            tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  1:497099/1‑139 (MQ=255)            tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  2:733445/1‑139 (MQ=255)            tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  2:468170/1‑139 (MQ=255)            tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  1:272327/1‑139 (MQ=255)            tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  2:722513/1‑139 (MQ=255)                      cccTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAg                                                                                                   >  1:467946/1‑139 (MQ=255)                      cccTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAg                                                                                                   >  2:194728/1‑139 (MQ=255)                           ttttAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGaa                                                                                                <  1:33249/137‑1 (MQ=255)                           ttttAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGaa                                                                                                <  2:30503/137‑1 (MQ=255)                           ttttAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGaa                                                                                                <  2:140940/137‑1 (MQ=255)                           ttttAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGaa                                                                                                <  1:93186/137‑1 (MQ=255)                                     aGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGaa                                                                                     >  2:718720/1‑138 (MQ=255)                                     aGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGaa                                                                                     >  1:580159/1‑138 (MQ=255)                                     aGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGaa                                                                                     >  1:699329/1‑138 (MQ=255)                                      ggCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGaata                                                                                   <  1:72691/139‑1 (MQ=255)                                                    tCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAActg                                                                      <  2:272327/138‑1 (MQ=255)                                                     cGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAActgct                                                                    >  2:77553/1‑139 (MQ=255)                                                      gTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAActgct                                                                    <  1:722280/138‑1 (MQ=255)                                                               ttctCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGc                                                            >  2:548859/1‑137 (MQ=255)                                                                 ctcGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCaa                                                          <  1:722513/137‑1 (MQ=255)                                                                 ctcGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCaa                                                          <  2:172966/137‑1 (MQ=255)                                                                 ctcGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAg                                                         >  1:723364/1‑138 (MQ=255)                                                                 ctcGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAg                                                         >  1:354597/1‑138 (MQ=255)                                                                 ctcGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGa                                                        <  2:723364/139‑1 (MQ=255)                                                                                    aTCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTgcgcgc                                      >  1:47140/1‑138 (MQ=255)                                                                                          cagcaTACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTAc                               <  1:712249/139‑1 (MQ=255)                                                                                          cagcaTACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTAc                               <  1:468170/139‑1 (MQ=255)                                                                                             caTACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCaa                                                          <  1:349585/109‑1 (MQ=255)                                                                                             caTACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCaa                                                          >  2:349585/1‑109 (MQ=255)                                                                                             caTACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGc                            >  2:81650/1‑139 (MQ=255)                                                                                             caTACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGc                            <  1:733445/139‑1 (MQ=255)                                                                                                aCGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCta                          >  1:534915/1‑138 (MQ=255)                                                                                                       aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGt                                                                 >  2:71993/1‑92 (MQ=255)                                                                                                       aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGt                                                                 <  1:71993/92‑1 (MQ=255)                                                                                                       aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGa                                                        <  1:541254/101‑1 (MQ=255)                                                                                                       aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGa                                                        >  2:541254/1‑101 (MQ=255)                                                                                                       aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGa                                                        <  2:77400/101‑1 (MQ=255)                                                                                                       aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGa                                                        >  1:77400/1‑101 (MQ=255)                                                                                                       aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGaa                  >  2:33972/1‑139 (MQ=255)                                                                                                       aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGaa                  >  2:664596/1‑139 (MQ=255)                                                                                                         ttcttcCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTgcgcg                                       <  2:239149/116‑1 (MQ=255)                                                                                                         ttcttcCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTgcgcg                                       >  1:239149/1‑116 (MQ=255)                                                                                                           cttcCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGaa                                                                                                <  1:111566/57‑1 (MQ=255)                                                                                                           cttcCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGaa                                                                                                >  2:111566/1‑57 (MQ=255)                                                                                                           cttcCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACg                >  2:129812/1‑137 (MQ=255)                                                                                                           cttcCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACg                <  2:699329/137‑1 (MQ=255)                                                                                                           cttcCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACg                <  1:129812/137‑1 (MQ=255)                                                                                                           cttcCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCt              >  2:590733/1‑139 (MQ=255)                                                                                                           cttcCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCt              >  2:115521/1‑139 (MQ=255)                                                                                                                 ccTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCg        >  2:416960/1‑139 (MQ=255)                                                                                                                       tatGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATaaa  <  2:580159/139‑1 (MQ=255)                                                                                                                       tatGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATaaa  <  1:194728/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                      ATCTTAAACAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAA  >  NZ_CP009273/2974790‑2975047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑