Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,058,644	Δ1 bp	coding (483/1404 nt)	yihO ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,058,644	0	A	.	100.0%	226.3 / NA	52	coding (483/1404 nt)	yihO	MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (26/26);  total (26/26)

CAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATC  >  NZ_CP009273/4058515‑4058776                                                                                                                                  |                                                                                                                                     caGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGc                                                                                                                            >  2:120851/1‑139 (MQ=255)    catcatAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCgg                                                                                                                          >  1:128801/1‑138 (MQ=255)    catcatAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCgg                                                                                                                          >  2:440522/1‑138 (MQ=255)    catcatAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCgg                                                                                                                          >  1:713220/1‑138 (MQ=255)    catcatAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCgg                                                                                                                          >  1:493787/1‑138 (MQ=255)              aTAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAg                                                                                                               >  1:489685/1‑139 (MQ=255)                      gccAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTgcgcgc                                                                                                       <  2:420663/139‑1 (MQ=255)                       ccAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTgcgcgcg                                                                                                      <  2:239629/139‑1 (MQ=255)                                aaCATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCgg                                                                                             >  1:427483/1‑139 (MQ=255)                                   aTAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGtt                                                                                          <  1:440522/139‑1 (MQ=255)                                           cGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTaa                                                                                  <  1:400735/139‑1 (MQ=255)                                                  gCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCgg                                                                           >  2:181721/1‑139 (MQ=255)                                                           aCGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCg                                                                   >  1:306905/1‑138 (MQ=255)                                                           aCGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGc                                                                  <  2:128801/139‑1 (MQ=255)                                                            cGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTgcgcgcg                                                                                                      >  2:589452/1‑102 (MQ=255)                                                            cGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTgcgcgcg                                                                                                      <  1:589452/102‑1 (MQ=255)                                                            cGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCa                                                                 >  1:250000/1‑139 (MQ=255)                                                            cGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCa                                                                 >  1:720603/1‑139 (MQ=255)                                                            cGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCa                                                                 >  2:452525/1‑139 (MQ=255)                                                                  aGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGatcat                                                                     <  2:555955/129‑1 (MQ=255)                                                                  aGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGatcat                                                                     >  1:555955/1‑129 (MQ=255)                                                                      aGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAg                                                        <  1:185762/138‑1 (MQ=255)                                                                      aGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAg                                                        <  2:489685/138‑1 (MQ=255)                                                                       gAAAAAATCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCa                                                      <  2:385699/139‑1 (MQ=255)                                                                       gAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCa                                                      <  1:188218/139‑1 (MQ=255)                                                                       gAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCa                                                      <  1:452525/139‑1 (MQ=255)                                                                                 aCTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGa                                            <  1:498630/139‑1 (MQ=255)                                                                                             tAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGt                                >  1:229215/1‑139 (MQ=255)                                                                                               aaCGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCAt                                                             <  1:719569/108‑1 (MQ=255)                                                                                               aaCGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCAt                                                             >  2:719569/1‑108 (MQ=255)                                                                                               aaCGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTtcatca                                              >  2:124946/1‑123 (MQ=255)                                                                                               aaCGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTtcatca                                              <  1:124946/123‑1 (MQ=255)                                                                                                aCGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTcc                              >  1:371827/1‑138 (MQ=255)                                                                                                 cGCCACGGTGCAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCaa                            <  1:545384/139‑1 (MQ=255)                                                                                                 cGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCaa                            <  2:713220/139‑1 (MQ=255)                                                                                                 cGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCaa                            <  2:449671/139‑1 (MQ=255)                                                                                                      cGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAAcatc                       <  2:178360/139‑1 (MQ=255)                                                                                                            aaaTCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAAc                 <  2:229215/139‑1 (MQ=255)                                                                                                                 aaTAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAgcgc            >  2:735034/1‑139 (MQ=255)                                                                                                                 aaTAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAgcgc            <  2:306905/139‑1 (MQ=255)                                                                                                                   tAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAg                                                        <  2:745225/93‑1 (MQ=255)                                                                                                                   tAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAg                                                        >  1:745225/1‑93 (MQ=255)                                                                                                                   tAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCaa                            <  2:348869/121‑1 (MQ=255)                                                                                                                   tAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCaa                            <  2:208341/121‑1 (MQ=255)                                                                                                                   tAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCaa                            >  1:348869/1‑121 (MQ=255)                                                                                                                   tAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCaa                            >  1:208341/1‑121 (MQ=255)                                                                                                                   tAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCgg          >  1:512996/1‑139 (MQ=255)                                                                                                                        cTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGa                                            <  1:728567/100‑1 (MQ=255)                                                                                                                        cTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGa                                            >  2:728567/1‑100 (MQ=255)                                                                                                                           tGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGt         >  2:381599/1‑132 (MQ=255)                                                                                                                           tGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGt         <  1:381599/132‑1 (MQ=255)                                                                                                                           tGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAAtc  >  1:258381/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                     CAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATC  >  NZ_CP009273/4058515‑4058776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑