Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,632,424	(A)7→6	intergenic (‑58/+21)	BW25113_RS08135 ← / ← BW25113_RS25620	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,632,418	0	A	.	100.0%	121.0 / NA	28	intergenic (‑52/+27)	BW25113_RS08135/BW25113_RS25620	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (12/16);  total (12/16)

TTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCA  >  NZ_CP009273/1632286‑1632546                                                                                                                                     |                                                                                                                                 tttATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTaaaaaac                                                                                                                            >  1:111223/1‑138 (MQ=255) tttATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTaaaaaac                                                                                                                            >  1:137349/1‑138 (MQ=255)          tcCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGAtttt                                                                                                                  >  2:10121/1‑139 (MQ=255)             ggTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGAttttttt                                                                                                               <  1:674382/139‑1 (MQ=255)             ggTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGAttttttt                                                                                                               <  2:154448/139‑1 (MQ=255)                        gAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTAAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTAc                                                                                                    <  1:365593/139‑1 (MQ=255)                          aaCCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCg                                                                                                  <  2:111223/139‑1 (MQ=255)                          aaCCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCg                                                                                                  <  1:483126/139‑1 (MQ=255)                          aaCCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCg                                                                                                  <  2:137349/139‑1 (MQ=255)                          aaCCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCg                                                                                                  <  1:216354/139‑1 (MQ=255)                          aaCCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCg                                                                                                  <  2:491158/139‑1 (MQ=255)                               tGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGtt                                                                                                      >  1:622669/1‑130 (MQ=255)                               tGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGtt                                                                                                      <  2:622669/130‑1 (MQ=255)                                gCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTAt                                                                                            >  1:304776/1‑139 (MQ=255)                                    ccATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGa                                                                                        <  1:10121/139‑1 (MQ=255)                                         aCAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATc                                                                                   >  2:449365/1‑139 (MQ=255)                                                  tgtgtTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCg                                                                                                  <  2:728918/115‑1 (MQ=255)                                                  tgtgtTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCg                                                                                                  >  1:728918/1‑115 (MQ=255)                                                  tgtgtTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGaa                                                                            >  1:181090/1‑137 (MQ=255)                                                     gtTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGa                                                                        >  1:308128/1‑138 (MQ=255)                                                                aTTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCaaaa                                                            <  2:304776/139‑1 (MQ=255)                                                                   tGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCaaaaaa                                                          <  1:254567/138‑1 (MQ=255)                                                                                            aCTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCAc                                >  2:343029/1‑139 (MQ=255)                                                                                                 aGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACttttt                           >  2:550448/1‑139 (MQ=255)                                                                                                 aGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACttttt                           >  1:119165/1‑139 (MQ=255)                                                                                                 aGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACttttt                           >  1:109196/1‑139 (MQ=255)                                                                                                        gCTGGCACTACCACTGCTGGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATc                     >  2:491541/1‑138 (MQ=255)                                                                                                                     ctgctgGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGc       <  2:308128/139‑1 (MQ=255)                                                                                                                        ctgGTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCt      <  2:119165/137‑1 (MQ=255)                                                                                                                           gTTCAGAGTT‑AAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCa  <  1:402183/138‑1 (MQ=255)                                                                                                                                     |                                                                                                                                 TTTATGATCTCCGGTCAGAGATGGAAACCCTGCGGCCATTACAGTAAGGTGTGTTTTTTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCA  >  NZ_CP009273/1632286‑1632546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑