| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,209,063 | +C | coding (719/732 nt) | mlrA → | HTH‑type transcriptional regulator MlrA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,209,060 | 1 | . | C | 96.9% | 113.5 / ‑2.8 | 32 | coding (716/732 nt) | mlrA | HTH‑type transcriptional regulator MlrA |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (15/16); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01 | |||||||||||
GCCCACTCGCTCAATCAACTACGCCCTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTT‑CCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATT > NZ_CP009273/2208930‑2209195 | gCCCACTCGCTCAATCAACTACGCCCTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTT‑ccccactc > 1:61791/1‑134 (MQ=255)gCCCACTCGCTCAATCAACTACGCCCTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTT‑ccccact < 2:158489/138‑5 (MQ=255) cACTCGCTCAATCAACTACGCCCTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCgg > 1:739611/1‑138 (MQ=255) aatcaaCTACGCCCTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTT‑CCCACTCGGCATTTAATGAt < 2:376651/139‑1 (MQ=255) atcaaCTACGCCCTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGAt < 1:237494/139‑1 (MQ=255) aCGCCCTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTaa > 2:2920/1‑139 (MQ=255) gCCCTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAAc > 1:225099/1‑138 (MQ=255) cccTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACaa < 2:229423/139‑1 (MQ=255) aaCTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATgcg < 2:618850/139‑1 (MQ=255) aaCTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATgcg < 2:61791/139‑1 (MQ=255) ttCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAAt < 2:736553/116‑1 (MQ=255) ttCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAAt > 1:736553/1‑116 (MQ=255) aGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTa > 1:566224/1‑139 (MQ=255) gCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCg < 2:225099/138‑1 (MQ=255) gCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCg < 2:27976/138‑1 (MQ=255) gCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCg < 1:370173/138‑1 (MQ=255) gtgtggtgCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTaa > 1:693337/1‑138 (MQ=255) tggtgCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCttt < 1:758978/129‑1 (MQ=255) tggtgCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCttt > 2:758978/1‑129 (MQ=255) gtgCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAg > 2:12600/1‑139 (MQ=255) gtgCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAg > 1:601962/1‑139 (MQ=255) tGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGa < 2:349494/135‑1 (MQ=255) tGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGa > 1:349494/1‑135 (MQ=255) aaCCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATgcgc < 2:601962/139‑1 (MQ=255) gCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTa < 2:620017/139‑1 (MQ=255) cccAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTa > 1:8751/1‑138 (MQ=255) cagcagCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATgcg < 1:10236/80‑1 (MQ=255) cagcagCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATgcg > 2:10236/1‑80 (MQ=255) caACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAgg > 1:96274/1‑106 (MQ=255) caACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAgg < 2:96274/106‑1 (MQ=255) aCCAGTTGGCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGcc < 1:575106/138‑1 (MQ=255) ggCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGAt < 2:464981/46‑1 (MQ=255) ggCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGAt > 1:464981/1‑46 (MQ=255) ggCAAGAACAAGGCCATGATATTTTCCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGCcctgttcct > 2:482502/1‑139 (MQ=255) tttc‑CCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGCCCTGTTCCTGTTTATGGCGTTAGGCGGAAtt > 2:456963/1‑139 (MQ=255) | GCCCACTCGCTCAATCAACTACGCCCTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTT‑CCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATT > NZ_CP009273/2208930‑2209195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |