Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	297,613	(C)5→6	coding (672/690 nt)	paoA ←	aldehyde dehydrogenase iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	297,608	1	.	C	100.0%	159.8 / NA	43	E226G (GAG→GGG)	paoA	aldehyde dehydrogenase iron‑sulfur subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (20/23);  total (20/23)

TCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCT‑CCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGAC  >  NZ_CP009273/297471‑297740                                                                                                                                           |                                                                                                                                     tCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTAtct‑c                                                                                                                                     >  1:402668/1‑139 (MQ=255)         cAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCT‑ccccccgca                                                                                                                             <  1:190689/139‑5 (MQ=255)           gCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCgcagc                                                                                                                            <  1:198415/139‑1 (MQ=255)           gCTTCAGCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCgcagc                                                                                                                            <  2:662596/139‑1 (MQ=255)                           cagATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCa                                                                                                            <  2:741989/139‑1 (MQ=255)                            agATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCaa                                                                                                           <  1:7416/139‑1 (MQ=255)                            agATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCaa                                                                                                           <  2:196455/139‑1 (MQ=255)                              aTTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAg                                                                                                          >  1:244918/1‑138 (MQ=255)                                tGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGAt                                                                                                       >  2:185744/1‑139 (MQ=255)                                tGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGAt                                                                                                       >  1:337155/1‑139 (MQ=255)                                     ccgcccgcGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTa                                                                                                   <  2:94041/138‑1 (MQ=255)                                      cgcccgcGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGc                                                                                                 <  1:214760/139‑1 (MQ=255)                                        cccgcGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTa                                                                                                   >  1:520725/1‑135 (MQ=255)                                        cccgcGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTa                                                                                                   <  2:520725/135‑1 (MQ=255)                                         ccgcGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTa                                                                                              <  2:402668/139‑1 (MQ=255)                                                       gcgcCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGAc                                                                                <  2:773001/139‑1 (MQ=255)                                                                  gcgcTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCg                                                                     <  2:411784/139‑1 (MQ=255)                                                                       gAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCa                                                                 >  2:146175/1‑138 (MQ=255)                                                                       gAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCa                                                                 >  1:268635/1‑138 (MQ=255)                                                                        aGCGCTAAGTGCCGCCTCGGCTGGTGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCa                                                                 <  1:302017/137‑1 (MQ=255)                                                                        aGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCa                                                                 <  2:534200/137‑1 (MQ=255)                                                                        aGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCAt                                                                <  2:6454/138‑1 (MQ=255)                                                                        aGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATa                                                               >  1:517775/1‑139 (MQ=255)                                                                        aGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATa                                                               >  1:154385/1‑139 (MQ=255)                                                                         gcgcTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATAc                                                              <  1:161496/139‑1 (MQ=255)                                                                                   ccgccTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTCGCCGCTCATACGTTCACGGAt                                                    <  1:185744/139‑1 (MQ=255)                                                                                    cgccTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGc                                                                                                 >  2:657200/1‑93 (MQ=255)                                                                                    cgccTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGc                                                                                                 <  1:657200/93‑1 (MQ=255)                                                                                    cgccTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGAtc                                                   >  2:49213/1‑139 (MQ=255)                                                                                                    ttCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGttt                                   >  2:411587/1‑139 (MQ=255)                                                                                                         tCGTTCATAGGTAAACGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTg                                >  2:33119/1‑137 (MQ=255)                                                                                                                      aaCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCaa                                                                                                           <  2:266923/49‑1 (MQ=255)                                                                                                                      aaCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCaa                                                                                                           >  1:266923/1‑49 (MQ=255)                                                                                                                      aaCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAAt                 >  2:254369/1‑139 (MQ=255)                                                                                                                       aCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATg                                                                                                      <  2:749045/53‑1 (MQ=255)                                                                                                                       aCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATg                                                                                                      >  1:749045/1‑53 (MQ=255)                                                                                                                       aCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGtt                                                                                                    <  1:55851/55‑1 (MQ=255)                                                                                                                       aCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGtt                                                                                                    >  2:55851/1‑55 (MQ=255)                                                                                                                       aCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACcaca                                                                                     >  1:382574/1‑70 (MQ=255)                                                                                                                       aCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATc                >  1:358913/1‑139 (MQ=255)                                                                                                                       aCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATc                >  1:194389/1‑139 (MQ=255)                                                                                                                       aCGCCTTCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGCTAGCGTATGCACcaca                                                                                     <  2:382574/70‑1 (MQ=255)                                                                                                                             tCATGATTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGAGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACcgt          >  1:300265/1‑139 (MQ=255)                                                                                                                                  aTTTTATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACcgtgacg      <  2:337155/138‑1 (MQ=255)                                                                                                                                      tATCTCCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCgtgacgtgac  <  2:358913/138‑1 (MQ=255)                                                                                                                                           |                                                                                                                                     TCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAACGCCTTCATGATTTTATCT‑CCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGAC  >  NZ_CP009273/297471‑297740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑