| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 353,591 | 0 | T | C | 52.2% | 9.8 / 32.7 | 23 | K271E (AAA→GAA) | cynR | transcriptional regulator CynR |
| Reads supporting (aligned to +/- strand): ref base T (9/2); new base C (0/12); total (9/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.99e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCG‑CATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATC > NZ_CP009273/353454‑353712 | aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCG‑CATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTc > 2:372277/1‑139 (MQ=255) aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCGGTGTCAGCGACAACCGTGATTCATTTCCGCCAACAACCGCG‑CATTTATCCAACGCCAGGTGCAAAAATGCCGTCGCGGCGGCTGTCTGCCAGCTATTTTTccgc < 2:149940/139‑1 (MQ=17) tgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCG‑CATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACCAAACCGCCGTTCTCTCCAGCAGTGGCggg > 2:734010/1‑139 (MQ=255) tgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCG‑CATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggg > 2:61358/1‑139 (MQ=255) gCGGCTACCGTGATTCATTTCCGCCAACAACCGCG‑CATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggca > 1:544244/1‑135 (MQ=255) ccGTGTTTCATTCCCGCAAACACCCGCG‑CTTTTACCCAACGCCAGGTGAAAAAATGCCTCCGCGGCGGCTGTCTGCCAGCTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAAt < 1:401944/138‑1 (MQ=25) ctttCCGCCAACCACCCCGTCTTTTACCCACCGCCTGTTGCAAAAATGCTTTGGGGGCGGTGGTCGGCCAGCTTTTTACCCGCCGCAAAAAAGCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTaa < 2:398924/137‑1 (MQ=255) tcccGCCAACACCCGCC‑CTTTTACCCACCGCCATGTGAAAAAATCCCTGCGGGGGGGCTGTCTGCCAGTTATTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGc < 1:372277/135‑1 (MQ=255) ccccaaacaccCGCG‑TATTTATCCAACCCAATGTGAAAAAATGCTTTCGCGGCGGCTGTCTGCCAGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCg < 1:436079/133‑1 (MQ=255) ccccAACACCCGCC‑CATTTACCCACCGCCATGTGAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTc < 1:333120/137‑1 (MQ=38) ccAACAACCGCG‑CATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGG‑AAAAAATTTTAAACCCgcct > 1:637294/1‑136 (MQ=25) gcg‑cATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑G‑GAAAAACATTTAACCCCCCCTGTTGTGTgg > 1:286958/1‑139 (MQ=25) gcg‑cATTTACCCACCGCCATGTCAAAAAATGCCTTCGGGGCGGTTGTCTGCCAGCTATTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTgg < 2:651951/139‑1 (MQ=255) tacccACCGCTATGTGAAAAAATGCCTCCGCGGCGGCTGTCTGCAATTTATTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCg < 2:637294/135‑1 (MQ=255) ccAACCCCTTGTAAAAAAATCCCTCCGGGGGGGCTGTCTGCCAGTTTTTTTCCCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctgc < 2:223113/139‑1 (MQ=255) cGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGG‑G‑AAAAAACCTTTAACCCCCCCTGTTTTGTGGGAAACGCTGCTgg > 1:545796/1‑138 (MQ=25) cccATGTGCAAAAATCCTTTCGGGGCGCCTGCTTGCCAGCTATTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTa < 1:315916/138‑1 (MQ=255) ccttgtgCAAAAATCCCTTCGCGGCGGCTGTCTGCCAGCTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTaa < 1:703754/136‑1 (MQ=255) tgtgCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCGGA‑GAAAAAAACTTAATGCCCCTC‑TGTTGTGTGGGAAGCGCAGCTGGTTACa > 2:722682/1‑138 (MQ=25) tgtgCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑G‑AAAAAACTTTTAACCCGCCATGTTTTGTTGGGATAGCTGCCggggacc > 1:505468/1‑133 (MQ=25) tgCAAAAACGCTTCCGCGGCTGCTGCCCGCCCTCTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAt < 1:154155/138‑1 (MQ=255) atgcccTCCGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGaaa < 2:505468/135‑1 (MQ=255) tCTGCCAGCTATTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc < 1:584260/137‑1 (MQ=255) | ATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCG‑CATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATC > NZ_CP009273/353454‑353712 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |