Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273365,1630CT59.3% 16.6 / 29.1 27S218S (AGC→AGT) mhpB3‑carboxyethylcatechol 2,3‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base C (3/8);  new base T (7/9);  total (10/17)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTCCGGTGGGCTTTCCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGA  >  NZ_CP009273/365028‑365285
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ttCCGGTGGGCTTTCCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGcgcc                                                                                                                         >  2:339203/1‑139 (MQ=255)
  cgggggggTTTCCCCATCACCCGCGGGGCCCCGAACGGGGGAAGGCGGATGCCCATAGGCGCGACCGTCTGTGGGGGGGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGtgccg                                                                                                                        <  1:474811/133‑1 (MQ=255)
  ccGGTGGGCTTTCCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGtgccgc                                                                                                                       >  2:257049/1‑139 (MQ=255)
  ccGGTGGGCTTTCCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGcgccgc                                                                                                                       >  1:181492/1‑139 (MQ=255)
  ccGGAGGGCTTTCCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGtgccgc                                                                                                                       >  1:362873/1‑139 (MQ=255)
        ggCTTTCCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGTGCCGCTgag                                                                                                                   >  1:226610/1‑137 (MQ=255)
                    ggccgcgGGTGCCCGAACTGGAGAAAGCCGATCCCCATATGCGCGGCCGTGTGTGGGGGGGGGGGAAATTTTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGTGCCGCTGAGAAGTTTGTTGAg                                                                                                       <  1:79220/136‑1 (MQ=255)
                    ggccgcgGGGGCCCAAACGGGAAAGGGCGGATCCCCATAGGCGCGCCGGTCTGTGGGGGGGGGGGAAAGTTTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGa                                                                                                     <  1:208405/138‑1 (MQ=255)
                                   agatGGCAAAAGCGGACCCACATAGGCGCGTCCGTGTGTGGGGGGGGGGAAAAAATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGTGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCa                                                                                      <  2:614746/136‑1 (MQ=255)
                                         caaaaGCCGATGCCCATATGCGCGCCCGTCTGGGGGGGGGCGGGAAAGATTTCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCt                                                                                <  2:181492/137‑1 (MQ=255)
                                                cGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGTGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAAccc                                                                          >  2:750280/1‑138 (MQ=255)
                                                            gcgcgcCCGTCTGGTGGGGAGGGGGAAGATTTTCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGTGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAAc                                                             <  2:275678/139‑1 (MQ=255)
                                                            gcgcGGCCGTGTGTGGGGGAGGGGGAAAGTTTTCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAAc                                                             <  2:664497/139‑1 (MQ=255)
                                                            gcgcGACCGTTTGGGGGGGGGGGGAAAATATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAAc                                                             <  1:320365/139‑1 (MQ=255)
                                                               cgACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGTGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAg                                                          >  2:179613/1‑139 (MQ=255)
                                                                   cGTCTGTTGGGGAGCGGGACAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGTGCCGCTGAGTAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCa                                                      >  2:102348/1‑139 (MQ=255)
                                                                     tCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCAt                                                     >  2:519093/1‑138 (MQ=255)
                                                                                   ggAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGTGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAg                                        >  2:729025/1‑137 (MQ=255)
                                                                                       atttttCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGa                                  <  2:722483/136‑1 (MQ=255)
                                                                                         aTTTCCCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACg                                <  2:320918/139‑1 (MQ=255)
                                                                                            tgcccGCCAGTGAGCGCGAAGTGCGTCAGCAACGGGTGATTAGTGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCa                              <  2:276778/136‑1 (MQ=255)
                                                                                                   cAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGTGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGc                                             <  2:406348/116‑1 (MQ=255)
                                                                                                   cAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGTGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGc                                             >  1:406348/1‑116 (MQ=255)
                                                                                                   cAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGTGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGaa                      >  1:295767/1‑139 (MQ=255)
                                                                                                        aGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTggg                                                                  >  1:100334/1‑90 (MQ=255)
                                                                                                        aGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTggg                                                                  <  2:100334/90‑1 (MQ=255)
                                                                                                        aGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTgg                  <  2:280253/138‑1 (MQ=255)
                                                                                                            cgAATTGCGTCAGCAACGGGTGATTAGTGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGcc             <  1:179613/139‑1 (MQ=255)
                                                                                                            cgAATTGCGTCAGCAACGGGTGATTAGTGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGcc             <  1:564914/139‑1 (MQ=255)
                                                                                                              aagtGCGTCAGCAACAGGTGATTAGTGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGGTGAATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGCAGCAGGGACGCAGACATGAACTGGATGCCgg           <  2:748547/136‑2 (MQ=255)
                                                                                                                       aGCAACGGGTGATTAGTGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGa  <  1:750280/139‑1 (MQ=255)
                                                                                                                         cAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACg   >  2:129115/1‑136 (MQ=255)
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TTCCGGTGGGCTTTCCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGCGTCAGCAACGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGA  >  NZ_CP009273/365028‑365285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑