Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,145,0620GT52.9% 5.3 / 19.1 18D133Y (GAT→TAT) yegJDUF2314 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (4/4);  new base T (9/0);  total (14/4)
Fisher's exact test for biased strand distribution p-value = 2.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCA‑TTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTG  >  NZ_CP009273/2144948‑2145199
                                                                                                                   |                                                                                                                                          
gACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCA‑TTGATCCTTTATTGGTCACCTATCCaa                                                                                                                    <  1:7717/139‑1 (MQ=255)
 aCCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCA‑TTTAtt                                                                                                                                         >  2:559999/1‑116 (MQ=255)
 aCCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCA‑TTGATc                                                                                                                                         <  1:559999/117‑1 (MQ=255)
 aCCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACA‑TTTATCCTTTTTTTGTTAACTATCCaa                                                                                                                    >  1:15823/1‑138 (MQ=255)
            gTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACA‑TTGATTCTTTTTTTGTCATCCATTCAAaaaaagaaagcc                                                                                                        >  2:7717/1‑135 (MQ=255)
             ttACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTAACA‑TTTATTCTTTTTTTGTCACCTATTCAAAAAAAAAAAcccc                                                                                                       >  1:81396/1‑137 (MQ=255)
                       aGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACA‑ATTATCCCTTATTTGTGACCAATCCCAAAAAAGAAACAGAAGAGCTaa                                                                                               >  2:678105/1‑137 (MQ=255)
                        gAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCA‑TTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAagag                                                                                            <  1:490414/139‑1 (MQ=255)
                             tGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACC‑TTTTTTCTTTTTTTGTTTCCTACCCCAAAAAAAAAAACGACCGGCGCAGAGCaaaa                                                                                       >  2:643581/1‑139 (MQ=255)
                                         gAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCA‑TTTATTCTTTTTTTGTTACCTATCCACAAGAAGAAACCGACGAGCTAAAAGCAAAAATAAttttgtaa                                                                           >  2:536815/1‑133 (MQ=255)
                                         gAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACA‑TTTATCCTTTTTTTGTTACCTATCCAAAAAAAAAAACCGACGAGATAAAAGCAAAAATAGTTCGCtca                                                                           >  2:201859/1‑137 (MQ=255)
                                         gAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACA‑TTGTTTCTTTTTTTGTGACCTATTCCAAAGAAGAAGGCGAAAAGCAAAAAGCAAAAATAGGTTGCtta                                                                           >  2:93446/1‑137 (MQ=255)
                                                         gaTATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCA‑TTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTAtattta                                                           <  1:678105/139‑1 (MQ=255)
                                                                       gATGTATACGCGCGGGGATAAAATTTACGGCGGGTTAACCACTTCTTCTTTTTTTTGGCA‑CTCTCCCCAAAAAAAAGCCGGCGCACTAAGAGGAAAATTATTTTTCTTCTCATCCGCGATATTTTTTTTTTtaaaatgc                                             >  2:535497/1‑134 (MQ=255)
                                                                                    ggggATAAAATTTACGGCGGTTTTACCC‑TTTTTTCTTTTTTTTTCCCCCATCCCAAAAAAGAAGCCCCCGAGCCAAGAGCAAAATTAATTTTCTAATTAGCCGCTTTATTTTTTTTTTTAAATTGC‑GGGTGTAtttttt                               >  1:42595/1‑138 (MQ=255)
                                                                                       gATAAAATTTACGGCGGTTATACCA‑ATTATCCTTTTTTTGTGACCTATCCCAAAAAAAAAAACGACGAGCTAAGAGAAAAAATAATTTGCTAATCAGCCGGTATATTTATTTTTTTTAATGGCGGGTTGAAttttgttt                             >  1:516712/1‑133 (MQ=255)
                                                                                                                 ttGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTTAATTTTTGTCTTCAACCGCCATGAGAAACTCActct   >  2:588681/1‑139 (MQ=255)
                                                                                                                  tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTTAATTTTTTTCTTCAACCGCCATGAGAAACTCACTCTg  >  1:503444/1‑139 (MQ=255)
                                                                                                                   |                                                                                                                                          
GACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCA‑TTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTG  >  NZ_CP009273/2144948‑2145199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑