Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,380,3410GA56.7% 1.3 / 34.5 30intergenic (+31/‑72)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
Reads supporting (aligned to +/- strand):  ref base G (3/10);  new base A (17/0);  total (20/10)
Fisher's exact test for biased strand distribution p-value = 9.52e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.66e-05
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACAT‑GAAATTCAACGGAGG‑AAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAA  >  NZ_CP009273/2380210‑2380463
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aaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATg                                                                                                                       <  2:536922/139‑1 (MQ=255)
     tACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACat                                                                                                                  >  1:262283/1‑139 (MQ=255)
               ccGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTcc                                                                                                        <  1:724557/139‑1 (MQ=255)
                                  aTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGACAAGAAAAAAAGAGAAGAGAACTTTATTTTTTCTCATCTGTTTTTAGaaac                                                                                     >  2:384360/1‑136 (MQ=255)
                                      aaTTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGaa                                                                                  <  1:384360/138‑1 (MQ=255)
                                          aTCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAGGAAAAGCATATTTTTTTTTTTTTCATATGTTTTTAAATGGGaaaaaa                                                                              >  1:335052/1‑135 (MQ=255)
                                                               tGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCCGAAAAAACGAACTGCACATTTTTTTTTTCCTCCTTTGTTTTTAAATGCGAAAAAT‑GAAATTTAAAGGAGG‑AAaaat                                                       >  1:587175/1‑138 (MQ=255)
                                                                     gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCCGAAAAAAAGACATGCAAATTTTTTTTTTTCTCCTCTGTTTTTAAATGCGAAAAAA‑GAAAATTAACGGAGG‑GAAAATAAATggg                                                >  2:578410/1‑139 (MQ=255)
                                                                     gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGGCAAGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGAGAAAAAT‑GAAAATCAACGGAGG‑GAAAATAAATtgg                                                >  2:373154/1‑139 (MQ=255)
                                                                     gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTATTTTTCCTCCTCTGTTTTTAAATGCGAAACAT‑GAAAATCAAAGGAGG‑AAAAATAAATggg                                                >  1:284269/1‑139 (MQ=255)
                                                                     gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACAATTTATTTTTCCTCATCTGTTTTTAGATGCGGAAAAA‑GAAAATTAACGGGGG‑GAAAATAAATggg                                                >  1:192900/1‑139 (MQ=255)
                                                                                ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCCGAAAAAAAGAAATGCACATTTTATTTTTCCTCTTCTGTTTTTAAATGCGAAACAT‑GAAATTCAAAGGGGG‑GAAAATAAATGGGGATGATTgggt                                     >  2:227743/1‑137 (MQ=255)
                                                                                ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTGCAGAAAAAACGACATGCACAATTTTTTTTTCCTCATCTGTTTTAAGATGCGGAAAAT‑GAAATTCAAAGGGGG‑GAAAATAAATTGGGATGATTGGc                                      >  1:445024/1‑138 (MQ=255)
                                                                                ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATTCGAAAAAT‑TAAATTTAACGGAGG‑AAAAATAAATGGGGATGATTGGCt                                     >  1:29633/1‑139 (MQ=255)
                                                                                ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTACAGAAAAAGAGACATGCACATTTTATTTTTCCTCATCTGTTTTAAGAAGCGAAACAA‑GAAATTCAACGGGGG‑AAAAATAAATGGGAATGGTTggg                                      >  2:741702/1‑137 (MQ=255)
                                                                                ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGGAAAAA‑GAAATTCAAAGGAGG‑AAAAATAAATGGGAATGATTTGCt                                     >  1:132765/1‑139 (MQ=255)
                                                                                  gTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGa                                                                                                                           >  2:216080/1‑53 (MQ=255)
                                                                                  gTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGa                                                                                                                           <  1:216080/53‑1 (MQ=255)
                                                                                      taCAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACAT‑GAAATTCAACGGAGG‑AAAAATAAATGGGAATGATTGGCTATTTTg                               <  1:227743/139‑1 (MQ=255)
                                                                                      acagacaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACAT‑GAAATTCAACGGAGG‑AAAAATAAATGGGAATGATTGGCTATTTTg                               <  1:88041/135‑1 (MQ=255)
                                                                                           caGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACAATTTATTTTTCCCCATCTGTTTTAAAAAGCGAAAAAT‑GAAAATTAAAGGAGG‑AAAAATAAATGGGAATGATTTGCTTTTTTTCaaa                           >  1:291808/1‑136 (MQ=255)
                                                                                             gTAAATAATAAAAATTTCTCGAGGCGGGTCCCAAAAAAACAAGAATAACAATTTATTTTTTTTCATCTGTTTTTAAAAGCGAAAAAA‑GAAACTCAAAGGAGG‑GAAAATAAAAAGGAATGGTTGGGGATTTTTCAGaaaa                        >  2:18383/1‑138 (MQ=255)
                                                                                              tAAATAATAAAAATTTCTCGAGGCGGGTGCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAAAAT‑GAAAATTAACGGGGG‑GAAAATAAAAGGGGATGATTTGCTATTTTTCAGAAAtt                       >  1:717756/1‑139 (MQ=255)
                                                                                                aataatAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACATGCACATTTTATTTTTCCTCATCTGTTTTTAGATGCGGAAAAT‑GAAATTTAACGGAGG‑AAAAATAAATGGGGATGATTTGCTATTTTTCAGAAATTg                      >  1:268545/1‑138 (MQ=255)
                                                                                                 ataataAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACAT‑GAAATTCAACGGAGG‑AAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGAc                    <  2:717756/139‑1 (MQ=255)
                                                                                                     taaAAATTTCTCGAGGCGGGTAGCACAAAAGCGAGACAAAAAATTTTTTTTCCATCATCAGTTTTATGATGCAGAACATGGAAATTC‑ACGGAGGTAAAAAAAAATGGG‑TTGATTTGGTATTTTTTAGaaactatct                   >  1:546457/1‑130 (MQ=255)
                                                                                                     taaAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCATCTGTTTTAAGATGCGGAACAT‑GAAATTCAACGGAGG‑GAAAAAAAAATGGAATGATTGGGTATTTTTCAGAAATTGaactgt                >  2:88041/1‑134 (MQ=255)
                                                                                                       aaaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACAT‑GAAATTCAACGGAGG‑AAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGaa              <  2:29633/139‑1 (MQ=255)
                                                                                                                 gAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACAT‑GAAATTCAACGGAGG‑AAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAAcc    <  2:368202/139‑1 (MQ=255)
                                                                                                                   ggCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACAT‑GAAATTCAACGGAGG‑AAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCaa  <  2:262283/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                            
AACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACAT‑GAAATTCAACGGAGG‑AAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAA  >  NZ_CP009273/2380210‑2380463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑