Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	10,865	A→C	G164G (GGT→GGG)	msyB ←	acidic protein MsyB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	10,865	0	A	C	100.0%	84.9 / NA	24	G164G (GGT→GGG)	msyB	acidic protein MsyB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/10);  total (14/10)

CCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGT  >  NZ_CP009273/10738‑10968                                                                                                                                |                                                                                                        ccccATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCaa                                                                                              >  2:718107/1‑139 (MQ=255)      tttAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAAtt                                                                                         <  1:519693/139‑1 (MQ=255)             ccGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCttt                                                                                  >  1:244848/1‑139 (MQ=255)                      cAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATcagca                                                                          <  2:466990/138‑1 (MQ=255)                           ccTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGgca                                                                    <  1:718107/139‑1 (MQ=255)                                        cgccgcgcAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAg                                                       >  1:72510/1‑139 (MQ=255)                                        cgccgcgcAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAg                                                       >  2:435246/1‑139 (MQ=255)                                        cgccgcgcAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAg                                                       >  2:280907/1‑139 (MQ=255)                                        cgccgcgcAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAg                                                       <  2:215955/139‑1 (MQ=255)                                              gcagcaAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCAtt                                                 <  1:435246/139‑1 (MQ=255)                                                         tGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCAt                                      >  1:411511/1‑139 (MQ=255)                                                            ccAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCAtt                                                 <  2:347979/125‑1 (MQ=255)                                                            ccAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCAtt                                                 >  1:347979/1‑125 (MQ=255)                                                                 tACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACcgcg                              >  2:650691/1‑139 (MQ=255)                                                                        aTTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGa                        >  1:480028/1‑138 (MQ=255)                                                                          tgctgcGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGcagcag                                                                >  1:505747/1‑96 (MQ=255)                                                                          tgctgcGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGcagcag                                                                <  2:505747/96‑1 (MQ=255)                                                                               cGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTcc                  <  1:650691/137‑1 (MQ=255)                                                                               cGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTcc                  <  2:411511/137‑1 (MQ=255)                                                                               cGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTg                >  1:751872/1‑139 (MQ=255)                                                                                gTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGc               >  1:463707/1‑139 (MQ=255)                                                                                    gTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTAt           >  1:467655/1‑139 (MQ=255)                                                                                    gTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTAt           >  1:274766/1‑139 (MQ=255)                                                                                             aTGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGt  <  2:463707/139‑1 (MQ=255)                                                                                                                                |                                                                                                        CCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGT  >  NZ_CP009273/10738‑10968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑