| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 77,241 | T→G | S37S (TCA→TCC) | leuC ← | 3‑isopropylmalate dehydratase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 77,241 | 0 | T | G | 96.7% | 223.5 / ‑0.4 | 61 | S37S (TCA→TCC) | leuC | 3‑isopropylmalate dehydratase large subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (29/30); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
TGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTT > NZ_CP009273/77107‑77364 | tGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAgg > 2:278525/1‑139 (MQ=255)tGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAgg > 1:490562/1‑139 (MQ=255)tGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAgg > 1:485761/1‑139 (MQ=255) cgcCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCa > 2:653041/1‑139 (MQ=255) ggCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCgg > 1:2087/1‑139 (MQ=255) gCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt < 1:592723/139‑1 (MQ=255) gCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt < 2:490562/139‑1 (MQ=255) gCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt < 1:453025/139‑1 (MQ=255) gCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt < 1:278525/139‑1 (MQ=255) gCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt < 2:421909/139‑1 (MQ=255) gCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt < 1:632906/139‑1 (MQ=255) ttAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGa < 1:736201/139‑1 (MQ=255) tCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGatatat > 2:616900/1‑138 (MQ=255) ggTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGt > 1:123240/1‑139 (MQ=255) agaCGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGtt > 1:709748/1‑139 (MQ=255) agaCGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGtt > 1:286538/1‑139 (MQ=255) aCGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGtttt < 2:2087/139‑1 (MQ=255) gTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCg < 1:616900/139‑1 (MQ=255) ttGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCgg > 2:372641/1‑139 (MQ=255) ttGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCgg > 2:366099/1‑139 (MQ=255) ttGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCgg > 2:161094/1‑139 (MQ=255) tgtgATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGc > 1:552713/1‑139 (MQ=255) gtgATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCt > 1:497606/1‑139 (MQ=255) tgATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCtt < 2:286538/139‑1 (MQ=255) aTCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTc < 2:323488/82‑1 (MQ=255) aTCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTc > 1:323488/1‑82 (MQ=255) aTGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCg < 1:33333/102‑1 (MQ=255) aTGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCg > 2:33333/1‑102 (MQ=255) tGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt > 1:333408/1‑99 (MQ=255) tGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt < 2:333408/99‑1 (MQ=255) tGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTacac < 2:653947/139‑1 (MQ=255) tGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTaca > 1:265864/1‑138 (MQ=255) tGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTaca > 1:153682/1‑138 (MQ=255) tGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTaca > 1:189544/1‑138 (MQ=255) tGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTaca > 2:463910/1‑138 (MQ=255) tGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTaca > 1:108542/1‑138 (MQ=255) gCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGt < 1:653041/139‑1 (MQ=255) cGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt > 2:175198/1‑93 (MQ=255) cGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt < 1:175198/93‑1 (MQ=255) aaGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt > 2:795/1‑91 (MQ=255) aaGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt < 1:795/91‑1 (MQ=255) tACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGtat < 2:552713/139‑1 (MQ=255) ccGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGtata < 1:463910/138‑1 (MQ=255) gCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTc < 1:372641/139‑1 (MQ=255) cGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCt > 1:567915/1‑139 (MQ=255) cGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCt < 1:435151/139‑1 (MQ=255) aCCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCa < 2:68883/50‑1 (MQ=255) aCCGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCa > 1:68883/1‑50 (MQ=255) ccGTGGGCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAg > 2:637724/1‑139 (MQ=255) ggCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt < 2:274040/56‑1 (MQ=255) ggCGCGCAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGt > 1:274040/1‑56 (MQ=255) cgcAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCg > 2:364268/1‑54 (MQ=255) cgcAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCg < 1:364268/54‑1 (MQ=255) cgcAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTa < 2:265864/139‑1 (MQ=255) cgcAGACCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTa < 2:497606/139‑1 (MQ=255) aCCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTacac > 1:93103/1‑137 (MQ=255) aCCATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTacac < 2:93103/137‑1 (MQ=255) ccATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACAcccc < 2:567915/139‑1 (MQ=255) ccATCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACAcccc < 2:485761/139‑1 (MQ=255) aTCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCtt < 2:57757/139‑1 (MQ=255) aTCGAACGCCTGCGGGGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCtt < 2:153682/139‑1 (MQ=255) | TGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTT > NZ_CP009273/77107‑77364 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |