| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,221,311 | A→G | pseudogene (927/1436 nt) | mdtQ ← | multidrug resistance outer membrane protein MdtQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,221,311 | 0 | A | G | 97.9% | 165.2 / ‑6.0 | 47 | pseudogene (927/1436 nt) | mdtQ | multidrug resistance outer membrane protein MdtQ |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (18/28); minor base C (0/1); total (18/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CCTGCCGTAACGCCCATTTGCTGCGCGGAATGACGGAACAGATCGCTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGACTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAACGCGACCGGTTTAAGCTTCAATGACTTTGTCTGGTTATTT > NZ_CP009273/2221175‑2221444 | ccTGCCGTAACGCCCATTTGCTGCGCGGAATGACGGAACAGATCGCTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCt < 2:256216/139‑1 (MQ=255)ccTGCCGTAACGCCCATTTGCTGCGCGGAATGACGGAACAGATCGCTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCt < 1:230799/139‑1 (MQ=255)ccTGCCGTAACGCCCATTTGCTGCGCGGAATGACGGAACAGATCGCTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCt < 1:542342/139‑1 (MQ=255) gCCGTAACGCCCATTTGCTGCGCGGAATGACGGAACAGATCGCTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCaa > 2:250686/1‑139 (MQ=255) tgctgcGCGGAATGACGGAACAGATCGCTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGcgcc < 2:132978/138‑1 (MQ=255) cgcgGAATGACGGAACAGATCGCTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTg > 1:650242/1‑138 (MQ=255) cgcgGAATGACGGAACAGATCGCTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTg > 1:628739/1‑138 (MQ=255) gcgGAATGACGGAACAGATCGCTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCa < 1:173928/139‑1 (MQ=255) gAACAGATCGCTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCccgc < 1:250686/139‑1 (MQ=255) aGATCGCTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCggg < 2:761500/139‑1 (MQ=255) aGATCGCTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCggg < 2:360801/139‑1 (MQ=255) gATCGATTAAGGGCAACGCATCCGGTTGCAGGAAGGCCATCAGGTGGATGTCAGGATAAAATGCCTCTTTTGCCGCATCAATGGTGCTTAGCGTTGCCTGAACGTACCAGTTCGCCGCCTGCAAATCTGCCCGCCGGGc < 2:455748/139‑1 (MQ=255) gCTTAAGTGCAACGCATCCGGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGt < 2:37873/139‑1 (MQ=255) cTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTa < 2:313686/139‑1 (MQ=255) cTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTa < 1:314932/139‑1 (MQ=255) aaGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAg > 1:259729/1‑138 (MQ=255) aGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAgg < 2:303426/138‑1 (MQ=255) gTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAg < 1:374624/139‑1 (MQ=255) tGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAg > 1:584737/1‑138 (MQ=255) aaCGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTAcc > 2:157823/1‑139 (MQ=255) cGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTAccc > 1:210427/1‑138 (MQ=255) aaGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCt > 2:545777/1‑139 (MQ=255) aTCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTg < 2:29535/139‑1 (MQ=255) aTCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTg < 2:259729/139‑1 (MQ=255) tGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACttt > 1:208637/1‑139 (MQ=255) ggATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAAcg > 2:757168/1‑139 (MQ=255) ggATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAAcg > 1:339810/1‑139 (MQ=255) tAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAACGCGa < 2:210427/139‑1 (MQ=255) aaaTGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAACGCGAc < 2:584737/138‑1 (MQ=255) aaaTGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAACGCGAc < 2:254920/138‑1 (MQ=255) aaaTGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAACGCGAc < 1:757168/138‑1 (MQ=255) cGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCggg < 2:116925/74‑1 (MQ=255) cGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCggg > 1:116925/1‑74 (MQ=255) cGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGt < 2:299048/79‑1 (MQ=255) cGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGt > 1:299048/1‑79 (MQ=255) cGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTg < 1:723003/113‑1 (MQ=255) cGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTg > 2:723003/1‑113 (MQ=255) cGCTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAACGCGACCGGttt > 1:184523/1‑138 (MQ=255) cTTTTGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAACGCGACCGGTTTAAg < 2:208637/139‑1 (MQ=255) ttGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGt < 1:101348/74‑1 (MQ=255) ttGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGt > 2:101348/1‑74 (MQ=255) ttGCCGCATCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAACGCGACCGGTTTAAGc < 1:545777/137‑1 (MQ=255) tCAATGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAACGCGACCGGTTTAAGCTTCAATGACt < 2:184523/139‑1 (MQ=255) tGGTGCTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAACGCGACCGGTTTAAGCTTCAATGACTTTGt < 2:650242/139‑1 (MQ=255) cTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCggg < 1:145904/52‑1 (MQ=255) cTTAGCGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCggg > 2:145904/1‑52 (MQ=255) cGATGGCTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAACGCGACCGGTTTAAGCTTCAATGACTTTGTCTGGTTAttt > 2:22560/1‑139 (MQ=255) | CCTGCCGTAACGCCCATTTGCTGCGCGGAATGACGGAACAGATCGCTTAAGTGCAACGCATCCTGTTGCAGGAAGGCCATCAGGTTGATGTCAGGATAAAATGCCGCTTTTGCCGCATCAATGGTGCTTAGCGATGACTCAACGTACCAGTGCGCCGCCTGCAAATCTGCCCGCCGGGCCAGTAAGGAGTACCCCAGTTCATCAGGAAGCTGGCTTGCCACTTTCGGCAACGCGACCGGTTTAAGCTTCAATGACTTTGTCTGGTTATTT > NZ_CP009273/2221175‑2221444 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |