Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,237,080	G→C	R18G (CGA→GGA)	folE ←	GTP cyclohydrolase I FolE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,237,080	0	G	C	100.0%	143.5 / NA	40	R18G (CGA→GGA)	folE	GTP cyclohydrolase I FolE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (15/25);  total (15/25)

TCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGC  >  NZ_CP009273/2236951‑2237201                                                                                                                                  |                                                                                                                          tCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTaa                                                                                                                  <  1:96327/139‑1 (MQ=255)   aaaCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACg                                                                                                                <  2:681252/139‑1 (MQ=255)         tcatcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCa                                                                                                           <  2:154806/138‑1 (MQ=255)           atcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATg                                                                                                         >  1:761766/1‑138 (MQ=255)              aGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAAcc                                                                                                     <  1:535979/139‑1 (MQ=255)                ccAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAg                                                                                                   >  1:30036/1‑139 (MQ=255)                   ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAGGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGAGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGc                                                                                                >  2:242913/1‑139 (MQ=255)                   ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCATCTAACGCTTCATGAACCAGGGc                                                                                                <  2:214460/139‑1 (MQ=255)                   ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGc                                                                                                <  2:30036/139‑1 (MQ=255)                   ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGc                                                                                                <  2:225994/139‑1 (MQ=255)                   ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGc                                                                                                <  1:126409/139‑1 (MQ=255)                   ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGc                                                                                                >  1:326720/1‑139 (MQ=255)                   ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGc                                                                                                >  2:61123/1‑139 (MQ=255)                   ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGc                                                                                                <  2:651231/139‑1 (MQ=255)                     tCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCg                                                                                              <  1:61123/139‑1 (MQ=255)                     tCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCg                                                                                              <  2:29575/139‑1 (MQ=255)                     tCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCg                                                                                              <  1:482360/139‑1 (MQ=255)                     tCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCg                                                                                              <  2:94866/139‑1 (MQ=255)                        agaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGctt                                                                                           >  2:725180/1‑139 (MQ=255)                          aTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGcttct                                                                                         >  2:311271/1‑139 (MQ=255)                               gcagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACt                                                                                    <  2:389462/139‑1 (MQ=255)                                   cTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCa                                                                                                           <  2:9937/112‑1 (MQ=255)                                   cTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCa                                                                                                           >  1:9937/1‑112 (MQ=255)                                   cTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCg                                                                                              >  2:89853/1‑125 (MQ=255)                                   cTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCg                                                                                              <  1:89853/125‑1 (MQ=255)                                      cATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACg                                                                                                                >  2:232727/1‑104 (MQ=255)                                      cATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACg                                                                                                                <  1:232727/104‑1 (MQ=255)                                      cATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGAt                                                                             <  2:761766/139‑1 (MQ=255)                                                     atGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTtc                                                              >  1:443819/1‑139 (MQ=255)                                                           aGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGc                                                                                                >  1:745332/1‑99 (MQ=255)                                                           aGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGc                                                                                                <  2:745332/99‑1 (MQ=255)                                                               aTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGctt                                                                                           >  1:552512/1‑100 (MQ=255)                                                               aTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGctt                                                                                           <  2:552512/100‑1 (MQ=255)                                                                         tGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCa                                                                                                           <  2:1663/74‑1 (MQ=255)                                                                         tGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCa                                                                                                           >  1:1663/1‑74 (MQ=255)                                                                               tttCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCAttt                                                                      <  2:189058/105‑1 (MQ=255)                                                                               tttCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCAttt                                                                      >  1:189058/1‑105 (MQ=255)                                                                                                   aCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGttatta                <  1:17445/139‑1 (MQ=255)                                                                                                             gcAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTa      <  1:725180/139‑1 (MQ=255)                                                                                                                 cGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGc  <  1:311271/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                          TCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGC  >  NZ_CP009273/2236951‑2237201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑