Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,243,084 A→G L5P (CTC→CCC)  yeiE ← DNA‑binding transcriptional regulator YeiE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,243,0840AG100.0% 113.4 / NA 32L5P (CTC→CCC) yeiEDNA‑binding transcriptional regulator YeiE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/18);  total (14/18)

CAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGA  >  NZ_CP009273/2242951‑2243217
                                                                                                                                     |                                                                                                                                     
cAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTg                                                                                                                                  <  2:96657/139‑1 (MQ=255)
     gCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTg                                                                                                                                  <  1:100137/134‑1 (MQ=255)
     gCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTg                                                                                                                                  >  2:100137/1‑134 (MQ=255)
       aCGCCAATCTGCCCTTCCAGGTCGGTCAATGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCa                                                                                                                           <  2:716244/139‑1 (MQ=255)
       aCGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCa                                                                                                                           <  2:702183/139‑1 (MQ=255)
        cGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCAt                                                                                                                          <  2:493432/139‑1 (MQ=255)
          ccAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAg                                                                                                                        <  1:348490/139‑1 (MQ=255)
           cAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGt                                                                                                                       <  2:741631/139‑1 (MQ=255)
                  cccTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTAc                                                                                                                <  2:546612/139‑1 (MQ=255)
                  cccTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTAc                                                                                                                <  2:656026/139‑1 (MQ=255)
                   ccTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTAcc                                                                                                               <  2:553420/139‑1 (MQ=255)
                               gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGat                                                                                                   >  2:447009/1‑139 (MQ=255)
                               gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGat                                                                                                   >  2:221552/1‑139 (MQ=255)
                                              aCTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAatata                                                                                     >  1:703417/1‑138 (MQ=255)
                                                     aTTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTAcc                                                                                                               >  2:582210/1‑105 (MQ=255)
                                                     aTTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTAcc                                                                                                               <  1:582210/105‑1 (MQ=255)
                                                     aTTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAat                                                                             >  2:329371/1‑139 (MQ=255)
                                                          gACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTg                                                                                                                                  >  1:735856/1‑81 (MQ=255)
                                                          gACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTAc                                                                                                                >  1:558594/1‑99 (MQ=255)
                                                          gACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTAc                                                                                                                <  2:558594/99‑1 (MQ=255)
                                                          gACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACGGCCGGGGGGTg                                                                                                                                  <  2:735856/81‑1 (MQ=255)
                                                                                ccTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAAc                                                  >  2:749854/1‑139 (MQ=255)
                                                                                    ggtggtTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTa                                              >  2:97259/1‑139 (MQ=255)
                                                                                    ggtggtTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTa                                              >  2:289553/1‑139 (MQ=255)
                                                                                        gtTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGc                                           <  1:447009/138‑1 (MQ=255)
                                                                                         ttGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGccc                                         >  1:759939/1‑139 (MQ=255)
                                                                                         ttGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGccc                                         >  1:266700/1‑139 (MQ=255)
                                                                                                    ttCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGga                               <  1:309401/138‑1 (MQ=255)
                                                                                                      cAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGgaga                             <  2:703417/138‑1 (MQ=255)
                                                                                                                 cAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGa                    <  1:750811/136‑1 (MQ=255)
                                                                                                                  aaaaaCTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGaa                <  1:221552/139‑1 (MQ=255)
                                                                                                                                 ccGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGa  >  1:268919/1‑138 (MQ=255)
                                                                                                                                     |                                                                                                                                     
CAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGA  >  NZ_CP009273/2242951‑2243217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑