Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,247,045	C→T	A194T (GCA→ACA)	nupX ←	NupC/NupG family nucleoside CNT transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,247,045	0	C	T	100.0%	84.2 / NA	22	A194T (GCA→ACA)	nupX	NupC/NupG family nucleoside CNT transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/10);  total (12/10)

TTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAA  >  NZ_CP009273/2246910‑2247176                                                                                                                                        |                                                                                                                                    tttCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTgtgg                                                                                                                                   <  2:462095/138‑1 (MQ=255)   tCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCACCAGCAGATATTCCACAGGCACGCCCAGTgtggcg                                                                                                                                 <  1:265139/138‑1 (MQ=255)   tCGGGGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGAGGCCGCCAGCAGATATTCCACAGGCACGCCCAGTgtggcg                                                                                                                                 <  1:519305/138‑1 (MQ=255)           agagagGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTgtggcg                                                                                                                                 >  1:109073/1‑130 (MQ=255)           agagagGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTgtggcg                                                                                                                                 <  2:109073/130‑1 (MQ=255)                     ttaAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAAc                                                                                                              <  2:526068/139‑1 (MQ=255)                        aaGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCTGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAg                                                                                                           <  2:562118/139‑1 (MQ=255)                            aaaCCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAAt                                                                                                       >  1:387338/1‑139 (MQ=255)                            aaaCCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAAt                                                                                                       >  2:31583/1‑139 (MQ=255)                               ccTGCGAAGATTCCGTTGCAGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATc                                                                                                      >  2:401759/1‑137 (MQ=255)                                         ttCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCa                                                                                          <  1:677511/139‑1 (MQ=255)                                             gTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGc                                                                                                 <  2:583460/128‑1 (MQ=255)                                             gTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGc                                                                                                 >  1:583460/1‑128 (MQ=255)                                                     gCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCt                                                                              <  2:342022/139‑1 (MQ=255)                                                     gCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCt                                                                              <  1:548238/139‑1 (MQ=255)                                                              gCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAaataat                                                                     >  2:268524/1‑139 (MQ=255)                                                                                gCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGAcga                                                   >  1:559754/1‑139 (MQ=255)                                                                                gCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGAcga                                                   <  1:152559/139‑1 (MQ=255)                                                                                 ccAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGAcgat                                                  <  2:559754/139‑1 (MQ=255)                                                                                          gCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTAGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAgg                                         >  2:346592/1‑139 (MQ=255)                                                                                                      gccgccAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTg                             >  1:348705/1‑139 (MQ=255)                                                                                                             gcagATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAAt                      >  1:760516/1‑139 (MQ=255)                                                                                                                               cGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGa     >  1:764869/1‑138 (MQ=255)                                                                                                                                gCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGaaa   <  2:450706/139‑1 (MQ=255)                                                                                                                                 cccAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGAc                                  <  2:12500/107‑1 (MQ=255)                                                                                                                                 cccAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGAc                                  >  1:12500/1‑107 (MQ=255)                                                                                                                                 cccAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGaaaa  >  2:392382/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                    TTTCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAA  >  NZ_CP009273/2246910‑2247176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑