Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,253,834	A→G	I352I (ATT→ATC)	fruA ←	PTS fructose transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,253,834	0	A	G	100.0%	155.2 / NA	43	I352I (ATT→ATC)	fruA	PTS fructose transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/19);  total (24/19)

CGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGAC  >  NZ_CP009273/2253702‑2253962                                                                                                                                     |                                                                                                                                 cGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGgatgat                                                                                                                             >  1:460215/1‑138 (MQ=255)   cccccAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAg                                                                                                                          >  1:27892/1‑139 (MQ=255)   cccccAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAg                                                                                                                          >  1:499652/1‑139 (MQ=255)               ccGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAg                                                                                                              <  2:460215/139‑1 (MQ=255)               ccGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAg                                                                                                              <  2:288637/139‑1 (MQ=255)                  cATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcgc                                                                                                           >  2:703791/1‑139 (MQ=255)                           tCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATAct                                                                                                  <  1:703791/139‑1 (MQ=255)                             cccATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATActct                                                                                                >  1:348709/1‑139 (MQ=255)                                aTGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCtgtg                                                                                             >  1:334582/1‑139 (MQ=255)                                     cTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGt                                                                                        >  1:588381/1‑139 (MQ=255)                                      tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGc                                                                                           <  2:560347/135‑1 (MQ=255)                                      tGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGc                                                                                           >  1:560347/1‑135 (MQ=255)                                        cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCGCCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcg                                                                                                            <  1:568670/116‑1 (MQ=255)                                        cAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGCAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcg                                                                                                            >  2:568670/1‑116 (MQ=255)                                               tGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCg                                                                              <  2:256208/139‑1 (MQ=255)                                               tGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCg                                                                              <  2:628925/139‑1 (MQ=255)                                                                aaTGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCa                                                             <  2:588381/139‑1 (MQ=255)                                                                 aTGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGAt                                                                                                                       >  1:122765/1‑80 (MQ=255)                                                                 aTGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGAt                                                                                                                       <  2:122765/80‑1 (MQ=255)                                                                  tGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAg                                                                                                              >  2:79549/1‑88 (MQ=255)                                                                  tGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAg                                                                                                              <  1:79549/88‑1 (MQ=255)                                                                      aGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTATCAATTGCGTACTGATTAACTTCGCAATGTaa                                                       >  2:389711/1‑139 (MQ=255)                                                                                    cGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGcc                                         <  2:348709/139‑1 (MQ=255)                                                                                    cGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGcc                                         <  2:221386/139‑1 (MQ=255)                                                                                    cGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGcc                                         <  1:450777/139‑1 (MQ=255)                                                                                      aTCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTaa                                                       <  2:42593/123‑1 (MQ=255)                                                                                      aTCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTaa                                                       >  1:42593/1‑123 (MQ=255)                                                                                        cAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGc                                      >  2:764141/1‑138 (MQ=255)                                                                                                   tcGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATaccac                           >  1:92864/1‑138 (MQ=255)                                                                                                   tcGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATaccac                           >  1:572053/1‑138 (MQ=255)                                                                                                     gCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCaa                        >  1:371859/1‑139 (MQ=255)                                                                                                      ccAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAAt                       <  1:99323/139‑1 (MQ=255)                                                                                                         gaccgaccACCAGACTGGAAATTAGCGGGATGATCAGGATCGGCTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTAATGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATg                      >  1:576305/1‑137 (MQ=255)                                                                                                          accgaccACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGc                                                                                           >  2:334212/1‑67 (MQ=255)                                                                                                          accgaccACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGc                                                                                           <  1:334212/67‑1 (MQ=255)                                                                                                              accaccAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGc                  >  1:147925/1‑136 (MQ=255)                                                                                                              accaccAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGc                  >  1:766975/1‑136 (MQ=255)                                                                                                              accaccAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGc                  >  1:480545/1‑136 (MQ=255)                                                                                                                 accaGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAAc            <  1:764141/139‑1 (MQ=255)                                                                                                                      aCTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTg       >  1:234748/1‑139 (MQ=255)                                                                                                                          gAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCaataa                                 >  2:278439/1‑109 (MQ=255)                                                                                                                          gAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCaataa                                 <  1:278439/109‑1 (MQ=255)                                                                                                                          gAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGa   >  2:592774/1‑139 (MQ=255)                                                                                                                           aaaTTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGAc  <  2:92864/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                 CGCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGAC  >  NZ_CP009273/2253702‑2253962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑