Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,378,215 A→G D329D (GAT→GAC)  yfbK ← VWA domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,378,2150AG100.0% 149.9 / NA 42D329D (GAT→GAC) yfbKVWA domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/21);  total (21/21)

CTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTT  >  NZ_CP009273/2378081‑2378342
                                                                                                                                      |                                                                                                                               
cTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGt                                                                                                                             >  1:748870/1‑139 (MQ=255)
   tAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCaa                                                                                                                          >  2:103261/1‑139 (MQ=255)
        gCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGcc                                                                                                                      >  1:731886/1‑138 (MQ=255)
         ccGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCa                                                                                                                     <  2:330969/138‑1 (MQ=255)
                    aTCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCa                                                                                                         >  2:572467/1‑139 (MQ=255)
                    aTCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCa                                                                                                         <  2:554751/139‑1 (MQ=255)
                      cGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCAcc                                                                                                       >  2:728569/1‑139 (MQ=255)
                        gCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGt                                                                                                     >  1:346605/1‑139 (MQ=255)
                          aaTTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCa                                                                                                   <  1:103261/139‑1 (MQ=255)
                                                ttGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCAc                                                                                                        <  2:526004/112‑1 (MQ=255)
                                                ttGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCAc                                                                                                        >  1:526004/1‑112 (MQ=255)
                                                 tGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCa                                                                                                   <  2:536096/116‑1 (MQ=255)
                                                 tGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCa                                                                                                   >  1:536096/1‑116 (MQ=255)
                                                   tAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATg                                                                           <  1:473902/138‑1 (MQ=255)
                                                   tAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATg                                                                           <  2:220991/138‑1 (MQ=255)
                                                         cTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCa                                                                                                   >  1:756277/1‑108 (MQ=255)
                                                         cTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCa                                                                                                   <  2:756277/108‑1 (MQ=255)
                                                                 cACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAAc                                                            >  2:268077/1‑139 (MQ=255)
                                                                    gCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAAcccc                                                         >  2:333432/1‑139 (MQ=255)
                                                                      cAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCtt                                                       >  2:476795/1‑139 (MQ=255)
                                                                        aaCGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATgcc                                                                         <  1:614469/119‑1 (MQ=255)
                                                                        aaCGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATgcc                                                                         >  2:614469/1‑119 (MQ=255)
                                                                                  gagTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCa                                                                                                   >  1:318975/1‑83 (MQ=255)
                                                                                  gagTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCa                                                                                                   <  2:318975/83‑1 (MQ=255)
                                                                                   agTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGt                                                                                                                             <  2:177788/56‑1 (MQ=255)
                                                                                   agTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGt                                                                                                                             >  1:177788/1‑56 (MQ=255)
                                                                                    gTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGAt                                         <  1:213524/139‑1 (MQ=255)
                                                                                    gTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGAt                                         <  1:651921/139‑1 (MQ=255)
                                                                                        cacCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGc                                     <  1:572467/139‑1 (MQ=255)
                                                                                             gACTCCCGCTGTTTTTTGACCATTGATTCAGTCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGtt                                >  2:465170/1‑139 (MQ=255)
                                                                                             gACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCa                                                                                                   >  1:474454/1‑72 (MQ=255)
                                                                                             gACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCa                                                                                                   <  2:474454/72‑1 (MQ=255)
                                                                                             gACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCct                                               <  2:469689/124‑1 (MQ=255)
                                                                                             gACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCct                                               >  1:469689/1‑124 (MQ=255)
                                                                                             gACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGtt                                >  2:311417/1‑139 (MQ=255)
                                                                                             gACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCACCTGCTGATAAGCCAGtt                                >  2:288000/1‑139 (MQ=255)
                                                                                                        ttttttGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCa                     <  2:699369/139‑1 (MQ=255)
                                                                                                        ttttttGACCACTGATTCAATCGATTTTGGGTCGTAAATTCCAACGTCAAAGTCACCGTCAGTAGCTAATAAAATGCGATGCATGCCGCCCTTAATAAACCCCTTCGTCACCTGCTGATAAGCCACTTCCAGCCCGGCa                     <  2:722597/139‑1 (MQ=255)
                                                                                                           tttGACCATTGATTCAACCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCAcc                   <  2:399589/138‑1 (MQ=255)
                                                                                                                     gATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTa         <  1:311417/138‑1 (MQ=255)
                                                                                                                      aTTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACt       <  2:718383/139‑1 (MQ=255)
                                                                                                                           aTCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCtt  >  1:198955/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                               
CTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTT  >  NZ_CP009273/2378081‑2378342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑