Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	97,990	A→C	T247P (ACC→CCC)	murC →	UDP‑N‑acetylmuramate‑‑L‑alanine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	97,990	0	A	C	100.0%	128.1 / NA	35	T247P (ACC→CCC)	murC	UDP‑N‑acetylmuramate‑‑L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/17);  total (18/17)

CTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGT  >  NZ_CP009273/97857‑98102                                                                                                                                      |                                                                                                                 ctaccAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCAc                                                                                                              >  1:173167/1‑138 (MQ=255)       gggCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTAc                                                                                                       >  2:146674/1‑139 (MQ=255)                  agaATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGa                                                                                            <  2:242305/139‑1 (MQ=255)                    aaTTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAg                                                                                          <  2:459325/139‑1 (MQ=255)                                aCTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGa                                                                                            >  2:683815/1‑125 (MQ=255)                                aCTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGa                                                                                            <  1:683815/125‑1 (MQ=255)                                aCTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGAcgtgc                                                                              >  1:610112/1‑139 (MQ=255)                                            tttCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGCAGATGCCGACGTGCGTGTAGAAGAt                                                                   >  2:133554/1‑138 (MQ=255)                                                        cTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTg                                                      <  2:173167/139‑1 (MQ=255)                                                                 tACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTc                                                                                                 <  2:388571/87‑1 (MQ=255)                                                                 tACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTc                                                                                                 >  1:388571/1‑87 (MQ=255)                                                                        gTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGAcgt                                                                                <  1:559609/97‑1 (MQ=255)                                                                        gTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGAcgt                                                                                >  2:559609/1‑97 (MQ=255)                                                                         tGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACtt                                      >  1:166036/1‑138 (MQ=255)                                                                            ggTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACg                                  <  2:559615/139‑1 (MQ=255)                                                                                aTGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACgctgc                              <  2:580392/139‑1 (MQ=255)                                                                                    gtgtTGATGATCCGGTGTTCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTgcg                          <  2:626987/139‑1 (MQ=255)                                                                                        tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTAc                                   <  2:543153/126‑1 (MQ=255)                                                                                        tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTAc                                   >  1:543153/1‑126 (MQ=255)                                                                                          atgatCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGa                    >  1:543798/1‑139 (MQ=255)                                                                                          atgagCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGa                    >  2:144127/1‑139 (MQ=255)                                                                                            gatCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGAc                   >  1:561912/1‑138 (MQ=255)                                                                                             atCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACa                  >  2:386879/1‑138 (MQ=255)                                                                                               ccGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACa                  >  1:466018/1‑136 (MQ=255)                                                                                                 ggTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAagag             >  1:588117/1‑139 (MQ=255)                                                                                                         gcgAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTgcg                          >  2:285599/1‑118 (MQ=255)                                                                                                         gcgAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTgcg                          <  1:285599/118‑1 (MQ=255)                                                                                                           gAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGcgcg   <  1:146674/139‑1 (MQ=255)                                                                                                           gAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGcgcg   <  2:610112/139‑1 (MQ=255)                                                                                                            aaTTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGt  <  2:65721/139‑1 (MQ=255)                                                                                                            aaTTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGt  <  2:166036/139‑1 (MQ=255)                                                                                                            aaTTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGt  <  2:561912/139‑1 (MQ=255)                                                                                                             aTTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGt  >  2:722071/1‑138 (MQ=255)                                                                                                                         aGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACa                  <  2:215596/110‑1 (MQ=255)                                                                                                                         aGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACa                  >  1:215596/1‑110 (MQ=255)                                                                                                                                      |                                                                                                                 CTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGT  >  NZ_CP009273/97857‑98102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑