Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,418,201	A→G	L171P (CTG→CCG)	hisM ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,418,201	0	A	G	100.0%	142.3 / NA	40	L171P (CTG→CCG)	hisM	ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/15);  total (25/15)

AAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAAC  >  NZ_CP009273/2418070‑2418336                                                                                                                                    |                                                                                                                                        aaGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTCGAGTGCGGCATCAg                                                                                                                                  >  1:519930/1‑139 (MQ=255)    agaTGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGAt                                                                                                                               <  2:628902/139‑1 (MQ=255)      atgatTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCAt                                                                                                                                     >  2:466951/1‑131 (MQ=255)      atgatTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCAt                                                                                                                                     <  1:466951/131‑1 (MQ=255)      atgatTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCa                                                                                                                             >  1:250859/1‑139 (MQ=255)      atgatTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCa                                                                                                                             >  1:765378/1‑139 (MQ=255)      atgatTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCa                                                                                                                             >  1:538897/1‑139 (MQ=255)         atTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACtt                                                                                                                          >  1:175287/1‑139 (MQ=255)                   gCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTa                                                                                                                >  1:506758/1‑139 (MQ=255)                   gCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTa                                                                                                                >  1:636815/1‑139 (MQ=255)                      ccGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGc                                                                                                             <  2:175287/139‑1 (MQ=255)                         cgGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCg                                                                                                                        >  1:133854/1‑125 (MQ=255)                         cgGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCg                                                                                                                        <  2:133854/125‑1 (MQ=255)                         cgGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCgg                                                                                                          >  2:98497/1‑139 (MQ=255)                         cgGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGACgg                                                                                                          >  1:610806/1‑139 (MQ=255)                                                    gATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCa                                                                                <  2:765378/138‑1 (MQ=255)                                                     aTACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAgg                                                                                  <  2:276658/135‑1 (MQ=255)                                                     aTACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAgg                                                                                  >  1:276658/1‑135 (MQ=255)                                                            gcggcgTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATg                                                                        >  2:600587/1‑138 (MQ=255)                                                               gcgTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCTGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGc                                                                    >  1:621270/1‑139 (MQ=255)                                                                 gTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGa                                                                  <  1:98497/139‑1 (MQ=255)                                                                         cgcgGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACAttt                                                           >  1:318610/1‑138 (MQ=255)                                                                          gcgGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGa                                                                                     >  2:81811/1‑111 (MQ=255)                                                                          gcgGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGa                                                                                     <  1:81811/111‑1 (MQ=255)                                                                          gcgGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACAtttt                                                          >  1:293451/1‑138 (MQ=255)                                                                                    ttCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCgag                                                <  2:666126/138‑1 (MQ=255)                                                                                    ttCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCgag                                                <  2:636815/138‑1 (MQ=255)                                                                                       agcagATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGc                                            >  2:728641/1‑139 (MQ=255)                                                                                              ccGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGaa                                              >  1:197696/1‑130 (MQ=255)                                                                                              ccGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGaa                                              <  2:197696/130‑1 (MQ=255)                                                                                                      gTGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTggcgg                             >  1:527501/1‑139 (MQ=255)                                                                                                       tGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATAcgcagcgca                                                                                       >  1:288910/1‑80 (MQ=255)                                                                                                       tGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATAcgcagcgca                                                                                       <  2:288910/80‑1 (MQ=255)                                                                                                       tGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTggcggc                            <  2:506758/139‑1 (MQ=255)                                                                                                       tGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTggcggc                            >  2:326164/1‑139 (MQ=255)                                                                                                       tGGCAGTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTggcggc                            >  1:523491/1‑139 (MQ=255)                                                                                                            gTAAATGCCAACGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCaa                       >  2:279411/1‑139 (MQ=255)                                                                                                                      aCGCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATg             <  2:44025/139‑1 (MQ=255)                                                                                                                        gCAGTAGAGTGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCg           <  2:610806/139‑1 (MQ=255)                                                                                                                                 tGCGGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAAc  >  1:58657/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                        AAGAGATGATTAAATAGAGCACCGCGGCAATGCCGAAGGCGGTAAAAGGTTGATACGTGGCGGCGTTAATATCGCGGGCTATTTTCAGCAGATCCGGCACCGTGGCAGTAAATGCCAACGCAGTAGAGTGCAGCATCAGGATCACTTCGTTGCTGTATGCCGGTAACGCAATACGCAGCGCAGAAGGCAAAATAATGCAGCGATACATTTTAAAAGTCGAGAAGCCATAGGCTCTGGCGGCTTCAATTTCCCCATGCGGAACCGAAC  >  NZ_CP009273/2418070‑2418336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑