Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,424,458	A→G	intergenic (‑216/+43)	cvpA ← / ← dedD	colicin V production protein/cell division protein DedD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,424,458	0	A	G	100.0%	89.6 / NA	25	intergenic (‑216/+43)	cvpA/dedD	colicin V production protein/cell division protein DedD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/16);  total (9/16)

CCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCGAGGCAT  >  NZ_CP009273/2424323‑2424592                                                                                                                                        |                                                                                                                                       ccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTggg                                                                                                                                      <  2:346886/138‑1 (MQ=255)     cccGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGa                                                                                                                                 >  2:445552/1‑139 (MQ=255)       cgcgCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGAt                                                                                                                                >  2:692387/1‑138 (MQ=255)         cgcATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGAt                                                                                                                                >  2:188371/1‑136 (MQ=255)          gcATCAATAAAAAGGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGa                                                                                                                            <  2:243819/139‑1 (MQ=255)                    aaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCg                                                                                                                  <  1:692387/139‑1 (MQ=255)                         gcgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCa                                                                                                             <  1:90194/139‑1 (MQ=255)                         gcgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCa                                                                                                             <  2:336556/139‑1 (MQ=255)                                   aaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGa                                                                                                   <  1:680574/139‑1 (MQ=255)                                    atatTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAc                                                                                                  <  2:233762/139‑1 (MQ=255)                                              ccATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAAtt                                                                                        <  1:466948/139‑1 (MQ=255)                                                aTCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCg                                                                                      <  1:445552/139‑1 (MQ=255)                                                     cTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGta                                                                                 <  1:423270/139‑1 (MQ=255)                                                     cTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGta                                                                                 <  2:470628/139‑1 (MQ=255)                                                         ttATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGc                                                                             >  1:590909/1‑139 (MQ=255)                                                           aTGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGcc                                                                            <  2:590909/138‑1 (MQ=255)                                                              ccTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCAt                                                                         <  1:229946/138‑1 (MQ=255)                                                               cTTTGCGGCATCGGGCAATGCGTTTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTa                                                                       <  2:457771/139‑1 (MQ=255)                                                                         tCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACt                                                             >  2:759420/1‑139 (MQ=255)                                                                                     tgtCGGATGCGGCGTAAACGCCTGATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAg                                                 <  2:27602/139‑1 (MQ=255)                                                                                       tCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGtt                                               >  2:617135/1‑139 (MQ=255)                                                                                                         ccTTATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCg                             <  2:701578/139‑1 (MQ=255)                                                                                                           ttATCCGACCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGaa                           >  2:412250/1‑139 (MQ=255)                                                                                                                  aCCTACGGTTCTACCCCTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCttt                      >  2:239571/1‑137 (MQ=255)                                                                                                                               ccccTGCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCGa       <  1:617135/139‑1 (MQ=255)                                                                                                                                    gCGTGGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCGAGGCAt  >  1:697603/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       CCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCGAACCTTTCAGCTTATCTTTCGAGGCAT  >  NZ_CP009273/2424323‑2424592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑