| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,434,438 | T→C | E216G (GAA→GGA) | fabB ← | beta‑ketoacyl‑ACP synthase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,434,438 | 0 | T | C | 94.7% | 123.3 / ‑0.7 | 38 | E216G (GAA→GGA) | fabB | beta‑ketoacyl‑ACP synthase I |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (14/22); total (15/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01 | |||||||||||
CCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGT > NZ_CP009273/2434303‑2434570 | ccGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTccct < 1:473414/139‑2 (MQ=255) aGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGgt > 1:339883/1‑139 (MQ=255) aaCGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGGTGTCg < 1:609924/139‑1 (MQ=255) aTTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGt < 1:632091/139‑1 (MQ=255) caccacGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGgtg < 1:113072/113‑1 (MQ=255) caccacGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGgtg > 2:113072/1‑113 (MQ=255) cacGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCgggg > 2:598447/1‑108 (MQ=255) cacGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCgggg < 1:598447/108‑1 (MQ=255) cacGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCAt > 1:99564/1‑139 (MQ=255) cgcgCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCgggg > 2:63216/1‑106 (MQ=255) cgcgCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCgggg < 1:63216/106‑1 (MQ=255) cgcgCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGGTGTCGtt < 2:159277/113‑1 (MQ=255) cgcgCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGGTGTCGtt > 1:159277/1‑113 (MQ=255) aGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAGCGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCa < 1:459445/139‑1 (MQ=255) aGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCa < 2:717142/139‑1 (MQ=255) aGCTCTCCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCa < 2:99564/139‑1 (MQ=255) ccgccgccAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGACTTCGCa > 1:573948/1‑110 (MQ=255) ccgccgccAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCa < 2:573948/110‑1 (MQ=255) ccgccgccAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGgtg < 1:322141/66‑1 (MQ=255) ccgccgccAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGgtg > 2:322141/1‑66 (MQ=255) ccgccgccAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTcgccgc > 2:477573/1‑139 (MQ=255) gccgccAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTcgccgccg > 2:252655/1‑139 (MQ=255) ccgccAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTcgccgccgc < 1:720150/139‑1 (MQ=255) cAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGc < 1:477573/139‑1 (MQ=255) aaaCCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGt < 1:402418/139‑1 (MQ=255) cgtcgtAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTcgccgccg < 1:526232/107‑1 (MQ=255) cgtcgtAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTcgccgccg > 2:526232/1‑107 (MQ=255) cgtcgtAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTg > 2:10582/1‑139 (MQ=255) cgtAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTtagt > 2:81530/1‑43 (MQ=255) cgtAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTtagt < 1:81530/43‑1 (MQ=255) gtAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGAt > 2:158644/1‑138 (MQ=255) gtAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGAt < 1:193542/138‑1 (MQ=255) gtAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGAt < 1:158644/138‑1 (MQ=255) gtAAGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGAt < 2:267255/138‑1 (MQ=255) aGTACGGGAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTg < 2:339883/138‑1 (MQ=255) gggAGGCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTAc > 2:532148/1‑139 (MQ=255) gCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGt < 2:712173/139‑1 (MQ=255) gCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGt < 1:252655/139‑1 (MQ=255) gCTTTTCCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGt < 2:117839/139‑1 (MQ=255) ttttcCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCa > 1:7837/1‑62 (MQ=255) ttttcCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCa < 2:7837/62‑1 (MQ=255) ttttcCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGt < 1:105963/137‑1 (MQ=255) ttttcCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGt > 2:105963/1‑137 (MQ=255) | CCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGT > NZ_CP009273/2434303‑2434570 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |