| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,435,986 | A→C | L248F (TTA→TTC) | mnmC → | bifunctional tRNA (5‑methylaminomethyl‑2‑thiouridine)(34)‑methyltransferase MnmD/FAD‑dependent 5‑carboxymethylaminomethyl‑2‑thiouridine(34) oxidoreductase MnmC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,435,986 | 0 | A | C | 95.8% | 82.7 / ‑3.5 | 24 | L248F (TTA→TTC) | mnmC | bifunctional tRNA (5‑methylaminomethyl‑2‑thiouridine)(34)‑methyltransferase MnmD/FAD‑dependent 5‑carboxymethylaminomethyl‑2‑thiouridine(34) oxidoreductase MnmC |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (7/16); total (8/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTAT > NZ_CP009273/2435851‑2436121 | gggCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCg < 1:692425/139‑1 (MQ=255) cGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCg > 2:535444/1‑131 (MQ=255) cGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCg < 1:535444/131‑1 (MQ=255) cGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTg > 2:597043/1‑139 (MQ=255) aCATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCgcgc < 2:336913/139‑1 (MQ=255) cATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGcc < 1:665178/139‑1 (MQ=255) ccGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTaa < 1:288072/136‑1 (MQ=255) ggTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCa < 2:428266/139‑1 (MQ=255) tCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCa < 1:356934/132‑1 (MQ=255) tCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCa > 2:356934/1‑132 (MQ=255) cgcgGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGgcagcagc > 2:314080/1‑138 (MQ=255) gCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGa > 1:397402/1‑139 (MQ=255) aCGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATcggcg < 1:317008/139‑1 (MQ=255) gATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCggt < 2:192505/139‑1 (MQ=255) aGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAgcgc > 1:42626/1‑139 (MQ=255) aGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAgcg < 1:695825/138‑1 (MQ=255) cAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGGTTGGCAGCGCGGTGGTGTCGccg > 2:9054/1‑137 (MQ=255) gAAATGCTTTGCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTAt < 2:42626/139‑1 (MQ=255) gCGGGGTGATGGAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGcgcg < 1:532735/138‑1 (MQ=255) gAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTa < 2:397402/138‑1 (MQ=255) gAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTa < 2:43916/138‑1 (MQ=255) gAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTa < 2:478393/138‑1 (MQ=255) gAACAGACATTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTa < 1:32528/138‑1 (MQ=255) aTTCCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGATTTGCAGGGCGGTGTTGTTGGGGGGGGTTTTTAGGCGCGGGTGGGAGGGGACGCttttt > 2:410869/1‑137 (MQ=255) | GGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTAT > NZ_CP009273/2435851‑2436121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |