Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,483,956 A→G S492P (TCA→CCA)  oxc ← oxalyl‑CoA decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,483,9560AG96.8% 102.8 / ‑6.3 31S492P (TCA→CCA) oxcoxalyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (17/13);  minor base T (1/0);  total (18/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

ATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAG  >  NZ_CP009273/2483820‑2484084
                                                                                                                                        |                                                                                                                                
atGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGgtggtg                                                                                                                                >  2:641979/1‑139 (MQ=255)
   gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGgtggtggtg                                                                                                                             >  1:32941/1‑139 (MQ=255)
            cgcgACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAgcg                                                                                                                     <  2:683697/138‑1 (MQ=255)
              cgACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCa                                                                                                                  <  1:641979/139‑1 (MQ=255)
                aCTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACt                                                                                                                <  2:32941/139‑1 (MQ=255)
                aCTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACt                                                                                                                <  1:622314/139‑1 (MQ=255)
                aCTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACt                                                                                                                <  1:376653/139‑1 (MQ=255)
                           gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAacac                                                                                                     >  2:286077/1‑139 (MQ=255)
                           gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAacac                                                                                                     >  2:467654/1‑139 (MQ=255)
                           gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAacac                                                                                                     >  2:57578/1‑139 (MQ=255)
                              gtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGt                                                                                                  <  1:467654/139‑1 (MQ=255)
                                 aaaGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGtct                                                                                                <  1:449456/138‑1 (MQ=255)
                                        gACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTg                                                                                          >  2:656435/1‑137 (MQ=255)
                                        gACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTg                                                                                          >  1:106886/1‑137 (MQ=255)
                                                  aTCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCAACAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCa                                                                              <  1:212714/139‑1 (MQ=255)
                                                                   tataGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGAt                                                             >  1:30320/1‑139 (MQ=255)
                                                                           aCGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCa                                                        <  2:89611/136‑1 (MQ=255)
                                                                           aCGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCa                                                        >  1:89611/1‑136 (MQ=255)
                                                                            cGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACt                                                                                                                >  2:414784/1‑79 (MQ=255)
                                                                            cGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACt                                                                                                                <  1:414784/79‑1 (MQ=255)
                                                                                          tCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGt                                                                                                  <  2:498263/79‑1 (MQ=255)
                                                                                          tCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGt                                                                                                  >  1:498263/1‑79 (MQ=255)
                                                                                                       cATACCTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAAt                         >  1:41127/1‑139 (MQ=255)
                                                                                                            cTTGCATGGTGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCa                    <  1:766914/139‑1 (MQ=255)
                                                                                                             ttGCATGGTGCAACAGATCCGTTGGTGTTGTTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGACATATCGACAAATCGCttcaatttcaat                   >  1:306813/1‑139 (MQ=255)
                                                                                                                     tGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTg            >  2:170558/1‑138 (MQ=255)
                                                                                                                     tGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTg            >  2:256956/1‑138 (MQ=255)
                                                                                                                     tGCAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTg            >  1:539105/1‑138 (MQ=255)
                                                                                                                       cAACAGATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGaaa         >  1:633141/1‑139 (MQ=255)
                                                                                                                            gATCCGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCaaa    <  2:633141/139‑1 (MQ=255)
                                                                                                                               ccGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAg  >  2:247041/1‑138 (MQ=255)
                                                                                                                               ccGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAg  >  2:691571/1‑138 (MQ=255)
                                                                                                                               ccGTTGGTGGTGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAg  >  1:257361/1‑138 (MQ=255)
                                                                                                                                        |                                                                                                                                
ATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAG  >  NZ_CP009273/2483820‑2484084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑