Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,544,265 A→T I104N (ATC→AAC)  yfeY ← RpoE‑regulated lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,544,2650AT94.7% 143.7 / 0.5 38I104N (ATC→AAC) yfeYRpoE‑regulated lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (19/17);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01

CACGATTATCATCACCATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCGGCA  >  NZ_CP009273/2544137‑2544396
                                                                                                                                |                                                                                                                                   
cACGATTATCATCACCATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCtt                                                                                                                            >  1:21760/1‑138 (MQ=255)
       atcatcACCATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGcc                                                                                                                     <  2:582476/138‑1 (MQ=255)
             aCCATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAAt                                                                                                               >  2:762116/1‑138 (MQ=255)
               cATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCAc                                                                                                            >  2:679469/1‑139 (MQ=255)
                       ttttGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCa                                                                                                    >  1:700779/1‑139 (MQ=255)
                        tttGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCa                                                                                                    <  1:645645/138‑1 (MQ=255)
                             cAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATGTCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGt                                                                                              <  1:469788/139‑1 (MQ=255)
                             cAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGt                                                                                              <  1:583809/139‑1 (MQ=255)
                                             tGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACtt                                                                               >  1:62716/1‑138 (MQ=255)
                                                       gTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGt                                                                                              >  2:72298/1‑113 (MQ=255)
                                                       gTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGt                                                                                              <  1:72298/113‑1 (MQ=255)
                                                        tCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGca                                                                   <  2:387689/139‑1 (MQ=255)
                                                         cGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGcac                                                                  >  1:410026/1‑139 (MQ=255)
                                                          gCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGcac                                                                  >  1:146321/1‑138 (MQ=255)
                                                                     tACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACG‑CAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTc                                                     >  2:194640/1‑139 (MQ=255)
                                                                          aTTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCgg                                                 >  2:5621/1‑139 (MQ=255)
                                                                           ttttAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGt                                                >  2:17312/1‑139 (MQ=255)
                                                                               aaCACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGtttt                                             >  1:31484/1‑138 (MQ=255)
                                                                                    cGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGt                                                                                              <  1:37054/84‑1 (MQ=255)
                                                                                    cGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGt                                                                                              >  2:37054/1‑84 (MQ=255)
                                                                                           agcagGAATATCGCTATCCAGCACG‑CAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTgcgc                                <  1:194640/138‑1 (MQ=255)
                                                                                            gcagGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCa                               <  1:537371/139‑1 (MQ=255)
                                                                                              agGAATATCGCTATCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTcgcg                                                  >  1:673427/1‑118 (MQ=255)
                                                                                              agGAATATCGCTATCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTcgcg                                                  <  2:673427/118‑1 (MQ=255)
                                                                                                     tCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCa                                           <  1:107551/118‑1 (MQ=255)
                                                                                                     tCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCa                                           >  2:107551/1‑118 (MQ=255)
                                                                                                          aTCCAGCACGTCAATGCGACTGTTCGTGCCTTGAt                                                                                                                         >  2:447253/1‑35 (MQ=255)
                                                                                                          aTCCAGCACGTCAATGCGACTGTTCGTGCCTTGAt                                                                                                                         <  1:447253/35‑1 (MQ=255)
                                                                                                          aTCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTgcg                                 <  2:29389/123‑1 (MQ=255)
                                                                                                          aTCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTgcg                                 >  1:29389/1‑123 (MQ=255)
                                                                                                               gCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAg            <  1:478762/139‑1 (MQ=255)
                                                                                                                cACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAgc           >  2:294105/1‑139 (MQ=255)
                                                                                                                   gTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCa        <  2:410026/139‑1 (MQ=255)
                                                                                                                   gTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCa        >  1:163530/1‑139 (MQ=255)
                                                                                                                    tCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCAt       <  2:31484/139‑1 (MQ=255)
                                                                                                                    tCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCAt       <  2:62716/139‑1 (MQ=255)
                                                                                                                       aTGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCgg    >  2:268827/1‑139 (MQ=255)
                                                                                                                          cGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCGGCa  <  2:613424/138‑1 (MQ=255)
                                                                                                                                |                                                                                                                                   
CACGATTATCATCACCATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCGGCA  >  NZ_CP009273/2544137‑2544396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑