| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,547,539 | A→G | S335P (TCC→CCC) | eutR ← | HTH‑type transcriptional regulator EutR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,547,539 | 0 | A | G | 94.3% | 109.2 / ‑0.9 | 35 | S335P (TCC→CCC) | eutR | HTH‑type transcriptional regulator EutR |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (20/13); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
GGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTA > NZ_CP009273/2547405‑2547652 | ggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGgaaa > 1:199013/1‑138 (MQ=255)ggAATATGATTATCAGCCAAAAGATAGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGgaaa > 1:301135/1‑138 (MQ=255) aGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATc > 1:399053/1‑139 (MQ=255) gCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTg > 1:567957/1‑139 (MQ=255) gcggcgTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGcc < 2:567957/139‑1 (MQ=255) gcgTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAgct < 2:431450/103‑1 (MQ=255) gcgTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAgct > 1:431450/1‑103 (MQ=255) gTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCa < 2:388442/139‑1 (MQ=255) tttATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATCGCCCCAGATGCCAGAATCCCCACTGCa > 2:396001/1‑139 (MQ=255) aaGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATggcg < 2:336491/138‑1 (MQ=255) gTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGt > 1:647427/1‑139 (MQ=255) aGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCtt > 2:111747/1‑139 (MQ=255) aGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCtt > 2:353924/1‑139 (MQ=255) ggATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCttt > 2:480771/1‑138 (MQ=255) gggTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATggcggc > 1:585627/1‑127 (MQ=255) gggTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATggcggc < 2:585627/127‑1 (MQ=255) gggTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATggcggc > 1:250140/1‑127 (MQ=255) gggTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATggcggc < 2:250140/127‑1 (MQ=255) gggTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAAt > 1:374676/1‑110 (MQ=255) gggTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAAt < 2:374676/110‑1 (MQ=255) ggTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGt < 2:560199/138‑1 (MQ=255) ccTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGc > 2:315071/1‑138 (MQ=255) cTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGa > 2:587689/1‑139 (MQ=255) tCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCa < 2:358546/86‑1 (MQ=255) tCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCa > 1:358546/1‑86 (MQ=255) tCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGcc > 1:479370/1‑92 (MQ=255) tCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGcc < 2:479370/92‑1 (MQ=255) cACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGAc > 1:312459/1‑139 (MQ=255) cTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATggcg < 1:276275/103‑1 (MQ=255) cTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATggcg > 2:276275/1‑103 (MQ=255) gCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGa > 1:598060/1‑81 (MQ=255) gCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGa < 2:598060/81‑1 (MQ=255) gCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAg > 1:284348/1‑139 (MQ=255) gCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGCGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAg > 1:373298/1‑139 (MQ=255) tGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAAt < 2:144587/75‑1 (MQ=255) tGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAAt > 1:144587/1‑75 (MQ=255) tGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTa < 1:353924/139‑1 (MQ=255) | GGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTA > NZ_CP009273/2547405‑2547652 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |