Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,676,053 T→G T18P (ACC→CCC)  yphG ← DUF5107 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,676,0530TG100.0% 162.5 / NA 44T18P (ACC→CCC) yphGDUF5107 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/23);  total (21/23)

CCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCAGTGTATTGAACAATCTGGCAATGTTTTCGCGG  >  NZ_CP009273/2675919‑2676184
                                                                                                                                      |                                                                                                                                   
ccACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCa                                                                                                                                 <  1:95940/139‑1 (MQ=255)
   cacCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAg                                                                                                                              >  2:427753/1‑139 (MQ=255)
         cTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCgg                                                                                                                         >  2:134262/1‑138 (MQ=255)
          tGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGa                                                                                                                       >  2:588192/1‑139 (MQ=255)
            ccAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGAtc                                                                                                                     >  1:191232/1‑139 (MQ=255)
                           tttCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGc                                                                                                      >  2:388103/1‑139 (MQ=255)
                            ttCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGc                                                                                                      <  1:199480/138‑1 (MQ=255)
                                 ctcgctcAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCacac                                                                                                 <  2:40856/138‑1 (MQ=255)
                                   cgctcAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACttt                                                                                              >  2:197228/1‑139 (MQ=255)
                                        aGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTAc                                                                                           <  1:254605/137‑1 (MQ=255)
                                        aGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTg                                                                                         <  2:167841/139‑1 (MQ=255)
                                                  tCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGt                                                                               <  1:588192/139‑1 (MQ=255)
                                                    aCGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCg                                                                                                                          >  2:357499/1‑94 (MQ=255)
                                                    aCGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCg                                                                                                                          <  1:357499/94‑1 (MQ=255)
                                                      gCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGccc                                                                           <  1:134262/139‑1 (MQ=255)
                                                      gCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGccc                                                                           <  1:354507/139‑1 (MQ=255)
                                                         gTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCa                                                                        <  2:191232/139‑1 (MQ=255)
                                                           aGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGc                                                                                                      <  1:333443/107‑1 (MQ=255)
                                                           aGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGc                                                                                                      >  2:333443/1‑107 (MQ=255)
                                                            gggAGAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAat                                                                      <  2:18177/138‑1 (MQ=255)
                                                                       cgcCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTa                                                                  >  1:619017/1‑131 (MQ=255)
                                                                       cgcCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTa                                                                  <  2:619017/131‑1 (MQ=255)
                                                                       cgcCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACa                                                          >  2:216860/1‑139 (MQ=255)
                                                                       cgcCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACa                                                          >  2:504455/1‑139 (MQ=255)
                                                                           ggACGAGCCCTGAGAAACGCGATTATCCAGGAACATAGGATGTATAGCCTGCGGCCCGAGTTCATAGGTCGGGATCTGAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACt                                                      <  2:161320/139‑1 (MQ=255)
                                                                                           aCGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTtgt                                      >  1:285715/1‑139 (MQ=255)
                                                                                           aCGCGATTTTCCAGGAACATGGGATGTATATCCTGCAGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTtgt                                      >  1:382577/1‑139 (MQ=255)
                                                                                            cgcgATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATgg                                                                                <  2:130376/96‑1 (MQ=255)
                                                                                            cgcgATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATgg                                                                                >  1:130376/1‑96 (MQ=255)
                                                                                               gATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCa                                  <  1:504455/139‑1 (MQ=255)
                                                                                               gATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCa                                  <  2:402385/139‑1 (MQ=255)
                                                                                               gATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCa                                  <  1:216860/139‑1 (MQ=255)
                                                                                                         gAACATGGGATGTATATCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCAGTGTATTGaa                        <  1:680076/139‑1 (MQ=255)
                                                                                                                       tatCCTGCGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCAGTGTATTGAACAATCTGGCAATGt          >  1:220714/1‑139 (MQ=255)
                                                                                                                              cGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAg                                                                                      <  1:666217/56‑1 (MQ=255)
                                                                                                                              cGGCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAg                                                                                      >  2:666217/1‑56 (MQ=255)
                                                                                                                               ggCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCa                                  >  2:386844/1‑107 (MQ=255)
                                                                                                                               ggCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCa                                  <  1:386844/107‑1 (MQ=255)
                                                                                                                               ggCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCAGTGTATTGAACAATCTGGCAATGTTTTcgcg   >  2:182708/1‑138 (MQ=255)
                                                                                                                                gCCCGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCAGTGTATTGAACAATCTGGCAATGTTTTCGCgg  >  1:474749/1‑138 (MQ=255)
                                                                                                                                 cccGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTgg                                    <  1:685308/103‑1 (MQ=255)
                                                                                                                                 cccGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTgg                                    >  2:685308/1‑103 (MQ=255)
                                                                                                                                 cccGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCa                                  <  2:32015/105‑1 (MQ=255)
                                                                                                                                 cccGGGTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCa                                  >  1:32015/1‑105 (MQ=255)
                                                                                                                                      |                                                                                                                                   
CCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCCTCAATATTAACAAGACATACTGAATTAAAAGATTTGTGGCAGTGTATTGAACAATCTGGCAATGTTTTCGCGG  >  NZ_CP009273/2675919‑2676184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑