| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,755,250 | T→C | R550R (AGA→AGG) | abpB ← | DEAD/DEAH box helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,755,250 | 0 | T | C | 90.5% | 58.6 / 0.1 | 21 | R550R (AGA→AGG) | abpB | DEAD/DEAH box helicase |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (11/8); total (13/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
TGCATAATCGATCTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGACACGGAAGTA > NZ_CP009273/2755121‑2755384 | tGCATAATCGATCTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAAt > 2:434760/1‑138 (MQ=255) cATAATCGATCTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGAt < 2:679075/139‑1 (MQ=255) tCGATCTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTg > 1:566872/1‑139 (MQ=255) aTCTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCa > 1:126685/1‑138 (MQ=255) cTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAg > 2:200500/1‑139 (MQ=255) aaTAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTcgc < 1:663282/139‑1 (MQ=255) tCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTc < 1:567706/138‑1 (MQ=255) tAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGaaa > 2:18650/1‑138 (MQ=255) aCATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCca < 2:419018/139‑1 (MQ=255) cGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCatcatc > 1:142256/1‑139 (MQ=255) aaCAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATg > 1:765870/1‑139 (MQ=255) aaCAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATg > 1:572792/1‑139 (MQ=255) aaCAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATg > 2:478706/1‑139 (MQ=255) gACCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTg > 2:292898/1‑138 (MQ=255) gACCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTg > 2:615686/1‑138 (MQ=255) aCCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTgca < 1:615686/139‑1 (MQ=255) aCCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTgca < 2:572792/139‑1 (MQ=255) aCCCCATTGCGACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTg > 1:683072/1‑137 (MQ=255) gACTGAACGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGaa < 2:765870/139‑1 (MQ=255) aaCGTATGGGGAACCAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCt < 1:504896/139‑1 (MQ=255) ccAGCCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGACACGGAAGTa > 2:235802/1‑139 (MQ=255) | TGCATAATCGATCTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTCTGGCAATGATCTTTGCAAAGGTCTTGAGGAAGTTTGTCGCGGGAGTCCAGTGAAACACTAACGAGGAAAATTCGTTATCGTCCATCATCTCGAACATGCTTTGCAGCATTTCGAACTGCTCGACACGGAAGTA > NZ_CP009273/2755121‑2755384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |