Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,756,916 T→C K533E (AAG→GAG)  abpA ← anti‑bacteriophage protein AbpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,756,9160TC97.3% 140.5 / ‑5.5 37K533E (AAG→GAG) abpAanti‑bacteriophage protein AbpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (18/18);  minor base G (1/0);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCCAAGTCATACGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTC  >  NZ_CP009273/2756783‑2757051
                                                                                                                                     |                                                                                                                                       
tcccAAGTCATACGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCtcat                                                                                                                                    >  1:109786/1‑139 (MQ=255)
tcccAAGTCATACGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCtcat                                                                                                                                    >  1:618530/1‑139 (MQ=255)
tcccAAGTCATACGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCtcat                                                                                                                                    >  1:509824/1‑139 (MQ=255)
  ccAAGTCATACGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCtcatc                                                                                                                                   <  1:406759/138‑1 (MQ=255)
           aCGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTgg                                                                                                                         >  2:766466/1‑139 (MQ=255)
                aCAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTaa                                                                                                                    <  1:38739/139‑1 (MQ=255)
                              caGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCg                                                                                                      >  2:108478/1‑139 (MQ=255)
                                 cTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCgggg                                                                                                   <  1:362947/139‑1 (MQ=255)
                                 cTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCgggg                                                                                                   <  2:280053/139‑1 (MQ=255)
                                       aaCCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGa                                                                                              <  1:108478/138‑1 (MQ=255)
                                           gCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAAt                                                                                         >  1:384885/1‑139 (MQ=255)
                                           gCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAAt                                                                                         <  1:220421/139‑1 (MQ=255)
                                           gCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAAt                                                                                         >  2:725289/1‑139 (MQ=255)
                                                       ttGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGAt                                                                             >  2:14658/1‑139 (MQ=255)
                                                                           aCCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCg                                                         <  1:14658/139‑1 (MQ=255)
                                                                           aCCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCg                                                         <  1:619362/139‑1 (MQ=255)
                                                                           aCCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCg                                                         <  2:387311/139‑1 (MQ=255)
                                                                                gCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATcc                                                    >  1:69220/1‑139 (MQ=255)
                                                                                     cTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGAt                                               <  1:725289/139‑1 (MQ=255)
                                                                                     cTATGTTTCGCCTGTTCATAGATCTCAGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCGAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGAt                                               >  1:742471/1‑139 (MQ=255)
                                                                                              gCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCt                                                                                                               <  1:593007/66‑1 (MQ=255)
                                                                                              gCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCt                                                                                                               >  2:593007/1‑66 (MQ=255)
                                                                                              gCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACg                                                                                  >  1:6886/1‑95 (MQ=255)
                                                                                              gCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACg                                                                                  <  2:6886/95‑1 (MQ=255)
                                                                                                  gTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGa                                                                                              <  2:273810/79‑1 (MQ=255)
                                                                                                  gTTCATAGATCTCTGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGa                                                                                              >  1:273810/1‑79 (MQ=255)
                                                                                                             tctGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCgggg                                                                                                   >  1:389039/1‑63 (MQ=255)
                                                                                                             tctGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCgggg                                                                                                   <  2:389039/63‑1 (MQ=255)
                                                                                                             tctGTCATACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGaa                       >  2:420942/1‑139 (MQ=255)
                                                                                                             tctGTCATACGACCTCTCTCACATGCTCATCAACTAATTGGGGTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGGGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCAGCGAATTTTTCGaa                       >  1:464303/1‑139 (MQ=255)
                                                                                                                    tACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCAcc                >  2:393231/1‑139 (MQ=255)
                                                                                                                    tACGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCAcc                >  2:522370/1‑139 (MQ=255)
                                                                                                                      cGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCg               <  1:647645/138‑1 (MQ=255)
                                                                                                                      cGACCTCTCTCACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGc              >  2:466419/1‑139 (MQ=255)
                                                                                                                               tcACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGtttct       <  2:509824/137‑1 (MQ=255)
                                                                                                                               tcACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGtttct       <  2:109786/137‑1 (MQ=255)
                                                                                                                               tcACCTCCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGtttct       <  1:393231/137‑1 (MQ=255)
                                                                                                                                  cctcctCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTtctc  <  2:395743/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
TCCCAAGTCATACGAAACAGGGTTAGACAACAGCTTGTTAACCGCAAACAGATAATTGAACGATGAGAAGTCCTCACCCTGCAGGCTATGTTTCGCCTGTTCATAGATCTCTGTCATACGACCTCTCTCACCTTCTCATCAACTAATTGGGTTAACCGCTCAAGACTCGGGGCTGGATAAATGAATACGTGGATGCAAAGATTCGCAAACGGCGAATCCTCGATGAGGTCAGCCGCGAATTTTTCGAACAGCACCGCTGTTTCTTTCTC  >  NZ_CP009273/2756783‑2757051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑