Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,786,008	T→G	G459G (GGT→GGG)	gabD →	NADP‑dependent succinate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,786,008	0	T	G	100.0%	165.3 / NA	45	G459G (GGT→GGG)	gabD	NADP‑dependent succinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/24);  total (21/24)

TGCCGCCTATTTCTACGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTCAGGCGATTCCCCG  >  NZ_CP009273/2785873‑2786143                                                                                                                                        |                                                                                                                                        tGCCGCCTATTTCTACGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGACGGGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTg                                                                                                                                      >  2:518960/1‑139 (MQ=255) tGCCGCCTATTTCTACGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTg                                                                                                                                      >  1:130352/1‑139 (MQ=255) tGCCGCCTATTTCTACGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTg                                                                                                                                      >  1:481554/1‑139 (MQ=255)  gccgccTATTTCTACGCCCGTGATTTAAGCCGTGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCt                                                                                                                                       <  2:228144/137‑1 (MQ=255)  gccgccTATTTCTACGCCCGTGATTTAAGCCGTGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTg                                                                                                                                      <  1:731924/138‑1 (MQ=255)          tttCTACGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTg                                                                                                                               <  1:169593/137‑1 (MQ=255)                cGCCCGTGATTTAAGCCGTGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTc                                                                                                                        >  2:735298/1‑138 (MQ=255)                cGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCg                                                                                                                       <  2:545444/139‑1 (MQ=255)                  cccGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGtt                                                                                                                         >  1:321500/1‑135 (MQ=255)                  cccGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGtt                                                                                                                         <  2:321500/135‑1 (MQ=255)                  cccGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCg                                                                                                                       >  1:555107/1‑137 (MQ=255)                    cGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAg                                                                                                                    >  1:225327/1‑138 (MQ=255)                     gTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAg                                                                                                                    >  2:68453/1‑137 (MQ=255)                     gTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAg                                                                                                                    <  1:68453/137‑1 (MQ=255)                             aGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCagc                                                                                                           <  1:536694/138‑3 (MQ=255)                                  cgTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGAt                                                                                                     >  2:264713/1‑139 (MQ=255)                                         cgcgTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAg                                                                                              <  1:496022/139‑1 (MQ=255)                                           cgTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGa                                                                                             >  2:680194/1‑138 (MQ=255)                                               ggCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATc                                                                                         >  1:24164/1‑138 (MQ=255)                                                            aGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                           <  1:735298/139‑1 (MQ=255)                                                            aGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                           <  2:24164/139‑1 (MQ=255)                                                            aGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                           <  2:130352/139‑1 (MQ=255)                                                              tACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGaaa                                                                                           <  2:174209/121‑1 (MQ=255)                                                              tACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGaaa                                                                                           >  1:174209/1‑121 (MQ=255)                                                                cGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTg                                                                                                                               >  1:231610/1‑83 (MQ=255)                                                                cGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTg                                                                                                                               <  2:231610/83‑1 (MQ=255)                                                                cGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGt                                                                       >  1:678130/1‑139 (MQ=255)                                                                 ggCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTc                                                                      <  2:555107/139‑1 (MQ=255)                                                                        tcgGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGa                                                                                             <  2:329825/109‑1 (MQ=255)                                                                        tcgGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGa                                                                                             >  1:329825/1‑109 (MQ=255)                                                                        tcgGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCaaa                                                                                      <  2:669533/116‑1 (MQ=255)                                                                        tcgGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCaaa                                                                                      >  1:669533/1‑116 (MQ=255)                                                                        tcgGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                           >  2:673775/1‑127 (MQ=255)                                                                        tcgGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCAt                                                                           <  1:673775/127‑1 (MQ=255)                                                                        tcgGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACt                                                               >  2:736535/1‑139 (MQ=255)                                                                                    ccGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGaa                                                   <  1:680194/139‑1 (MQ=255)                                                                                        cATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGaa                                               <  2:209949/139‑1 (MQ=255)                                                                                                 cAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATaa                                      <  1:736535/139‑1 (MQ=255)                                                                                                 cAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATaa                                      <  1:264713/139‑1 (MQ=255)                                                                                                  aaTGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGaa                                               >  2:591004/1‑129 (MQ=255)                                                                                                  aaTGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGaa                                               <  1:591004/129‑1 (MQ=255)                                                                                                  aaTGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATa                                       >  2:366841/1‑137 (MQ=255)                                                                                                                    ggcggcATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGcgccg                    <  1:365423/138‑1 (MQ=255)                                                                                                                      cggcATCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAg                 >  2:27477/1‑139 (MQ=255)                                                                                                                           tCAAAGCCTCGGGGCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTCAgg            <  2:481554/139‑1 (MQ=255)                                                                                                                                     ggggCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTCAGGCGATTCCCCg  >  2:289922/1‑139 (MQ=255)                                                                                                                                     ggggCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTCAGGCGATTCCCCg  <  1:518960/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TGCCGCCTATTTCTACGCCCGTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTCAGGCGATTCCCCG  >  NZ_CP009273/2785873‑2786143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑