Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,831,745 A→G I293I (ATT→ATC)  ascG ← DNA‑binding transcriptional regulator AscG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,831,7450AG100.0% 138.7 / NA 38I293I (ATT→ATC) ascGDNA‑binding transcriptional regulator AscG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/21);  total (17/21)

TGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCG  >  NZ_CP009273/2831610‑2831880
                                                                                                                                       |                                                                                                                                       
tGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGGTTTCGGCGGTGGGAAATCCCCGCCATCGAGCCTAAAAATCAGCCGTCCCATAATTTCCTGAATCATCTCAGTTACCGGGAtt                                                                                                                                      >  2:97136/1‑139 (MQ=255)
                      agtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGaa                                                                                                                <  2:44816/139‑1 (MQ=255)
                      agtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGaa                                                                                                                <  2:139184/139‑1 (MQ=255)
                      agtgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATAATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGaa                                                                                                                <  2:172634/139‑1 (MQ=255)
                        tgagtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACg                                                                                                              >  2:188216/1‑139 (MQ=255)
                           gtCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGgtg                                                                                                           >  2:695554/1‑139 (MQ=255)
                              gcggcgGATCAGTTCTCCGCTGAAAGTTTTCGGCGGTGAGAAATGCCCGCCATCGAGCATAGAAATCAGCCGTCCGATAATTACCTGAACCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGGGTa                                                                                                         <  2:459029/138‑1 (MQ=255)
                               cggcggATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAgg                                                                                                       <  2:687265/139‑1 (MQ=255)
                                ggcggATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAggg                                                                                                      >  1:613873/1‑139 (MQ=255)
                                  cggATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAggggg                                                                                                    <  1:166426/139‑1 (MQ=255)
                                        aGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAg                                                                                              <  2:670813/139‑1 (MQ=255)
                                                 cTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATatcat                                                                                     <  1:520299/139‑1 (MQ=255)
                                                    aaGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGgag                                                                                                                          <  2:158947/99‑1 (MQ=255)
                                                    aaGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGgag                                                                                                                          >  1:158947/1‑99 (MQ=255)
                                                    aaGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCg                                                                                   >  2:743278/1‑138 (MQ=255)
                                                                    agaAATCCCCGCCATCGAGCATAAAAATCAGCTGTCCAATAATTTCCTGCATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGaa                                                                                                                >  2:152640/1‑93 (MQ=255)
                                                                    agaAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCAGCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGaa                                                                                                                <  1:152640/93‑1 (MQ=255)
                                                                     gaAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACAcc                                                                 <  2:496876/139‑1 (MQ=255)
                                                                     gaAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACAcc                                                                 <  2:751427/139‑1 (MQ=255)
                                                                             cGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTgg                                                         <  1:188216/139‑1 (MQ=255)
                                                                                cATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAggggg                                                                                                    <  2:664634/93‑1 (MQ=255)
                                                                                cATCGAGCATAAAAATCAGCCGTCCAATAATTCCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAggggg                                                                                                    >  1:664634/1‑93 (MQ=255)
                                                                                  tCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCg                                                    <  2:229391/139‑1 (MQ=255)
                                                                                             aaTCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCgcgc                                         <  1:743278/139‑1 (MQ=255)
                                                                                                  gCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCgcgc                                         >  2:573480/1‑134 (MQ=255)
                                                                                                  gCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCgcgc                                         <  1:573480/134‑1 (MQ=255)
                                                                                                      tCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGaca                                                                   >  2:336197/1‑104 (MQ=255)
                                                                                                      tCCAATAATTTCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGaca                                                                   <  1:336197/104‑1 (MQ=255)
                                                                                                                tCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAggggg                                                                                                    <  2:281265/61‑1 (MQ=255)
                                                                                                                tCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAggggg                                                                                                    >  1:281265/1‑61 (MQ=255)
                                                                                                                tCCTGAATCATCTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCg                      >  2:145129/1‑139 (MQ=255)
                                                                                                                       tcatcTCAGTCACCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTAt               >  1:53682/1‑139 (MQ=255)
                                                                                                                                  aCCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACAcc                                                                 >  2:548738/1‑78 (MQ=255)
                                                                                                                                  aCCGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACAcc                                                                 <  1:548738/78‑1 (MQ=255)
                                                                                                                                   ccGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATAtcgtc   >  2:118124/1‑139 (MQ=255)
                                                                                                                                    cGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTgg                                                         >  2:387814/1‑84 (MQ=255)
                                                                                                                                    cGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTgg                                                         <  1:387814/84‑1 (MQ=255)
                                                                                                                                    cGGGATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATAtcgtcg  >  2:578978/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGCCGCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCG  >  NZ_CP009273/2831610‑2831880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑