Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,926,085	A→C	G96G (GGT→GGG)	fucO ←	lactaldehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,926,085	0	A	C	100.0%	114.3 / NA	32	G96G (GGT→GGG)	fucO	lactaldehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/20);  total (12/20)

GGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCACCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGCAT  >  NZ_CP009273/2925956‑2926209                                                                                                                                  |                                                                                                                             ggTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCaa                                                                                                                       >  2:154884/1‑137 (MQ=255)          tGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAgg                                                                                                            >  1:629740/1‑139 (MQ=255)                 aTCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCcg                                                                                                     <  2:698576/139‑1 (MQ=255)                    ggTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCgcgc                                                                                                  <  2:121977/139‑1 (MQ=255)                      tACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                <  1:154884/139‑1 (MQ=255)                      tACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                <  1:637536/139‑1 (MQ=255)                      tACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                <  1:36096/139‑1 (MQ=255)                             ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                >  2:607626/1‑132 (MQ=255)                             ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                <  1:607626/132‑1 (MQ=255)                             ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTAtt                                                                                           <  1:113661/137‑1 (MQ=255)                                       tCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACAcc                                                                               >  1:769993/1‑139 (MQ=255)                                            gAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGcc                                                                          <  1:358371/139‑1 (MQ=255)                                                gCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCcttc                                                                      >  1:303821/1‑139 (MQ=255)                                                 cccTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCct                                                                        >  2:668426/1‑136 (MQ=255)                                                 cccTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAAACCGCGCCGCTATTCTGGAATACACCGAGCCct                                                                        <  1:668426/136‑1 (MQ=255)                                                     tCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGCTAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTga                                                                 >  2:236531/1‑139 (MQ=255)                                                     tCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                <  2:175341/108‑1 (MQ=255)                                                     tCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                >  1:175341/1‑108 (MQ=255)                                                           cTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAg                                                           >  1:298665/1‑139 (MQ=255)                                                              cGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGt                                                          <  1:15329/137‑1 (MQ=255)                                                              cGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGt                                                          <  2:379436/137‑1 (MQ=255)                                                              cGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGt                                                          <  1:7201/137‑1 (MQ=255)                                                                        cAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTgttgggtt                                              <  2:770230/139‑1 (MQ=255)                                                                                  gttgttGCTGATAATTCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACg                                    <  1:598230/139‑1 (MQ=255)                                                                                                tGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGc                      >  2:166430/1‑139 (MQ=255)                                                                                                  ccAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGcc                     <  1:91784/138‑1 (MQ=255)                                                                                                  ccAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGcc                     <  1:236531/138‑1 (MQ=255)                                                                                                  ccAATCGCGTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGcc                     <  2:610044/138‑1 (MQ=255)                                                                                                         cTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAg             <  1:563186/139‑1 (MQ=255)                                                                                                           ttACAAGTATCCTGTGGAGAACCCCCACCAATAGCg                                                                                                                  <  2:56781/36‑1 (MQ=255)                                                                                                           ttACAAGTATCCTGTGGAGAACCCCCACCAATAGCg                                                                                                                  >  1:56781/1‑36 (MQ=255)                                                                                                                    tCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGcat  >  2:257709/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                             GGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCACCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGCAT  >  NZ_CP009273/2925956‑2926209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑