Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,111,922	T→C	N184D (AAT→GAT)	sslE ←	lipoprotein metalloprotease SslE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,111,922	0	T	C	100.0%	182.6 / NA	50	N184D (AAT→GAT)	sslE	lipoprotein metalloprotease SslE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/28);  total (22/28)

GTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGC  >  NZ_CP009273/3111786‑3112051                                                                                                                                         |                                                                                                                                  gTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCg                                                                                                                                 >  1:52891/1‑139 (MQ=255) gTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCg                                                                                                                                 >  2:238161/1‑139 (MQ=255) gTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCg                                                                                                                                 >  2:461607/1‑139 (MQ=255) gTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCg                                                                                                                                 <  2:284169/139‑1 (MQ=255) gTCCCAGCATTGCTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCAAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCGGTTCTGTATCCg                                                                                                                                 >  2:266510/1‑139 (MQ=255)   cGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGcc                                                                                                                               >  2:593879/1‑139 (MQ=255)     cagcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCgg                                                                                                                             <  2:334657/139‑1 (MQ=255)          ttGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGc                                                                                                                        <  1:238161/139‑1 (MQ=255)                    ccTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGa                                                                                                              <  1:497434/139‑1 (MQ=255)                               aCCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGc                                                                                                    >  1:682257/1‑138 (MQ=255)                                         tGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCAt                                                                                          <  1:604865/138‑1 (MQ=255)                                                ttCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTc                                                                                  >  2:227405/1‑139 (MQ=255)                                                  ccGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCtt                                                                                >  2:352491/1‑139 (MQ=255)                                                          aGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCg                                                                         >  2:724555/1‑138 (MQ=255)                                                            aTCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAg                                                                      <  1:65091/139‑1 (MQ=255)                                                                aTTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCg                                                                  >  1:213182/1‑139 (MQ=255)                                                                 tttGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGc                                                                 <  1:227405/139‑1 (MQ=255)                                                                             gCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc                                                     <  1:255513/139‑1 (MQ=255)                                                                             gCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc                                                     <  2:213182/139‑1 (MQ=255)                                                                              cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGa                                                                                                              <  2:212903/81‑1 (MQ=255)                                                                              cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGa                                                                                                              >  1:212903/1‑81 (MQ=255)                                                                              cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCtt                                                                                <  2:479974/111‑1 (MQ=255)                                                                              cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCtt                                                                                >  1:479974/1‑111 (MQ=255)                                                                              cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGcgcg                                                    >  2:69712/1‑139 (MQ=255)                                                                              cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGcgcg                                                    >  2:654986/1‑139 (MQ=255)                                                                               gAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCAt                                                                                          <  1:410759/100‑1 (MQ=255)                                                                               gAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCAt                                                                                          >  2:410759/1‑100 (MQ=255)                                                                                aGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTc                                                  >  1:323330/1‑139 (MQ=255)                                                                                  tCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCt                                                <  2:52891/139‑1 (MQ=255)                                                                                  tCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCt                                                <  2:430026/139‑1 (MQ=255)                                                                                   cGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc                                                     >  1:499312/1‑133 (MQ=255)                                                                                   cGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc                                                     <  2:499312/133‑1 (MQ=255)                                                                                   cGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCt                                                >  1:222313/1‑138 (MQ=255)                                                                                          tttACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCt                                         >  2:103825/1‑138 (MQ=255)                                                                                             aCTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTaaa                                      <  2:590173/138‑1 (MQ=255)                                                                                                ctcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGac                                  <  2:49996/139‑1 (MQ=255)                                                                                                   gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCgg                                                                                                                             <  2:591887/45‑1 (MQ=255)                                                                                                   gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCgg                                                                                                                             >  1:591887/1‑45 (MQ=255)                                                                                                   gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGac                                  <  1:123424/136‑1 (MQ=255)                                                                                                   gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGac                                  <  2:323330/136‑1 (MQ=255)                                                                                                   gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGac                                  <  1:623733/136‑1 (MQ=255)                                                                                                         cGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCt                                                <  2:359503/116‑1 (MQ=255)                                                                                                         cGAGGAGAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCt                                                >  1:359503/1‑116 (MQ=255)                                                                                                           tggagAACGTCAGACAAACCTGTTCTGTACCCGCCGGACAGCTGTGTCAGGCCGTTACCAGCGAAACCGCATTGCTTTTCTTTTCATCGGAACCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACCCTTTTTCAAc                       <  2:542841/138‑1 (MQ=255)                                                                                                           aggagAACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAAc                       <  2:222313/139‑1 (MQ=255)                                                                                                               gaACGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcgca                   <  1:528991/139‑1 (MQ=255)                                                                                                                  cGTCAGACAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcgcacg                 >  1:55570/1‑138 (MQ=255)                                                                                                                        aCAAACCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTa          <  1:630361/139‑1 (MQ=255)                                                                                                                            aCCTGTTCTGTATCCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcgcg      <  1:352491/139‑1 (MQ=255)                                                                                                                                 ttCTGTATCCGCCGGACAGCTGTTACTGGCCGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACCCTTTTTCAACCGCACGCAAGCTACGCGCAGc  <  1:524963/138‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  GTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGC  >  NZ_CP009273/3111786‑3112051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑