Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,143,589 A→C Y190S (TAC→TCC)  yghA → NADP(+)‑dependent aldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,143,5890AC100.0% 189.1 / NA 51Y190S (TAC→TCC) yghANADP(+)‑dependent aldehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/30);  total (21/30)

TGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAG  >  NZ_CP009273/3143455‑3143723
                                                                                                                                      |                                                                                                                                      
tGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAg                                                                                                                                    >  1:172916/1‑139 (MQ=255)
     aCCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAg                                                                                                                               >  1:123025/1‑139 (MQ=255)
               cGAACAGTTTCAAAAGTCCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCCATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTa                                                                                                                     <  1:221160/139‑1 (MQ=255)
               cGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTa                                                                                                                     <  2:529262/139‑1 (MQ=255)
               cGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTa                                                                                                                     <  1:385123/139‑1 (MQ=255)
               cGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCG‑AAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGc                                                                                                                          <  2:395513/133‑1 (MQ=255)
               cGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCG‑AAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGc                                                                                                                          >  1:395513/1‑133 (MQ=255)
                    aGTTTCAAAAGACCTTTGCCCTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGTAAGGTGCAAGCATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTggc                                                                                                                >  2:92908/1‑138 (MQ=255)
                    aGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGa                                                                                                                <  2:123025/139‑1 (MQ=255)
                    aGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGa                                                                                                                >  2:515991/1‑139 (MQ=255)
                    aGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGa                                                                                                                <  2:614696/139‑1 (MQ=255)
                             aGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGc                                                                                                       <  2:631232/139‑1 (MQ=255)
                                  tttGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACg                                                                                                   >  2:176358/1‑138 (MQ=255)
                                        aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAggcgg                                                                                            <  1:64337/139‑1 (MQ=255)
                                        aTTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAggcgg                                                                                            <  1:86479/139‑1 (MQ=255)
                                                    gcgcTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCGTCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACt                                                                                <  1:726972/139‑1 (MQ=255)
                                                     cgcTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTa                                                                               <  2:186996/139‑1 (MQ=255)
                                                          ttCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGcc                                                                          >  1:158125/1‑139 (MQ=255)
                                                                    cccAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAggcgg                                                                                            >  1:484995/1‑111 (MQ=255)
                                                                    cccAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAggcgg                                                                                            <  2:484995/111‑1 (MQ=255)
                                                                    cccAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTgg                                                                      <  1:16555/133‑1 (MQ=255)
                                                                    cccAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTgg                                                                      >  2:16555/1‑133 (MQ=255)
                                                                    cccAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTgg                                                                 >  1:454651/1‑138 (MQ=255)
                                                                         gAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTc                                                                                      >  1:333170/1‑112 (MQ=255)
                                                                         gAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTc                                                                                      <  2:333170/112‑1 (MQ=255)
                                                                               aTCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGc                                                                                                                          <  1:330021/70‑1 (MQ=255)
                                                                               aTCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGc                                                                                                                          >  2:330021/1‑70 (MQ=255)
                                                                                   cccTGCTACCGAAAGGTGCAAGTATCATCGCCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGga                                                 <  1:574159/139‑1 (MQ=255)
                                                                                       gCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGgaga                                               >  2:103238/1‑137 (MQ=255)
                                                                                           ccGACAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGCTTCTGAACTACAGCCGTGGCTTGGCAAAACCGGCCGCGGAGAAAGGTa                                         >  2:574159/1‑139 (MQ=255)
                                                                                             gAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTAtt                                       <  1:753772/139‑1 (MQ=255)
                                                                                                aGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCgg                                    <  1:444208/139‑1 (MQ=255)
                                                                                                aGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCgg                                    <  2:761237/139‑1 (MQ=255)
                                                                                                  gTGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGt                                  <  1:103238/139‑1 (MQ=255)
                                                                                                   tGCAAGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGt                                  >  1:698252/1‑138 (MQ=255)
                                                                                                      aaGTATCATCACCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAat                              <  2:698252/139‑1 (MQ=255)
                                                                                                             atcaCCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATg                                                                                                           <  1:314226/55‑1 (MQ=255)
                                                                                                             atcaCCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATg                                                                                                           >  2:314226/1‑55 (MQ=255)
                                                                                                             atcaCCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTAtt                                       <  1:72990/123‑1 (MQ=255)
                                                                                                             atcaCCACTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTAtt                                       >  2:72990/1‑123 (MQ=255)
                                                                                                                accacTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTAtt                                       >  2:291521/1‑120 (MQ=255)
                                                                                                                accacTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTAtt                                       <  1:291521/120‑1 (MQ=255)
                                                                                                                 ccacTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGcc                   <  2:454651/139‑1 (MQ=255)
                                                                                                                   acTTCGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAg                 >  2:696305/1‑139 (MQ=255)
                                                                                                                       cGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGc               <  2:172916/137‑1 (MQ=255)
                                                                                                                       cGTCAATCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGc               <  2:749237/137‑1 (MQ=255)
                                                                                                                           aaTCCAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGAt          <  2:526837/138‑1 (MQ=255)
                                                                                                                               cAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGa     <  1:765216/139‑1 (MQ=255)
                                                                                                                               cAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGa     >  2:49717/1‑139 (MQ=255)
                                                                                                                               cAGGCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGa     <  2:736067/139‑1 (MQ=255)
                                                                                                                                  gCATCCCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAg  >  2:239112/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                      
TGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCAATCCAGGCATACCAGCCAAGTCCGCATTTACTGGACTATGCGGCTACGAAGGCGGCGATTCTGAACTACAGCCGTGGCTTGGCAAAACAGGTCGCGGAGAAAGGTATTCGGGTGAATATTGTCGCGCCAGGCCCGATCTGGACAG  >  NZ_CP009273/3143455‑3143723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑