Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,246,701 A→G I291I (ATT→ATC)  yhaJ ← DNA‑binding transcriptional regulator YhaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,246,7010AG100.0% 120.7 / NA 33I291I (ATT→ATC) yhaJDNA‑binding transcriptional regulator YhaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/17);  total (16/17)

GCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAG  >  NZ_CP009273/3246565‑3246834
                                                                                                                                        |                                                                                                                                     
gCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGAt                                                                                                                                     >  2:645770/1‑139 (MQ=255)
        ggTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCa                                                                                                                             <  1:645770/139‑1 (MQ=255)
                tGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAg                                                                                                                     >  2:665355/1‑139 (MQ=255)
                  gcagcaATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGa                                                                                                                   >  2:402607/1‑139 (MQ=255)
                        aTCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                                                             <  1:665355/139‑1 (MQ=255)
                              aCGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCTCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTccc                                                                                                        <  2:108907/138‑1 (MQ=255)
                                       aTCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGt                                                                                               >  2:693130/1‑138 (MQ=255)
                                       aTCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGt                                                                                               >  2:602593/1‑138 (MQ=255)
                                              gTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGc                                                                                       <  1:602593/139‑1 (MQ=255)
                                                  ggTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAgg                                                                                   >  2:386434/1‑139 (MQ=255)
                                                   gTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                                                  <  1:334605/139‑1 (MQ=255)
                                                   gTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                                                  <  1:665962/139‑1 (MQ=255)
                                                   gTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                                                  <  2:461594/139‑1 (MQ=255)
                                                    ttCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                                                  >  2:31771/1‑138 (MQ=255)
                                                             aTCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCataataat                                                                         <  1:31771/138‑1 (MQ=255)
                                                                           gCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGt                                                          <  1:433589/139‑1 (MQ=255)
                                                                              atatTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                                                             >  1:568126/1‑85 (MQ=255)
                                                                              atatTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                                                             <  2:568126/85‑1 (MQ=255)
                                                                                    cGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGAtt                                                                                                                 <  2:243450/75‑1 (MQ=255)
                                                                                    cGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGAtt                                                                                                                 >  1:243450/1‑75 (MQ=255)
                                                                                    cGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCa                                                                                                     >  2:17171/1‑87 (MQ=255)
                                                                                    cGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCa                                                                                                     <  1:17171/87‑1 (MQ=255)
                                                                                              tattaACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTgacgac                                        <  1:693130/138‑1 (MQ=255)
                                                                                                               attattTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCg                       >  2:179359/1‑138 (MQ=255)
                                                                                                                 tattTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                                                  >  2:318460/1‑77 (MQ=255)
                                                                                                                 tattTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                                                  <  1:318460/77‑1 (MQ=255)
                                                                                                                      tCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTgacgac                                        >  2:140899/1‑114 (MQ=255)
                                                                                                                      tCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTgacgac                                        <  1:140899/114‑1 (MQ=255)
                                                                                                                        cGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                                                             <  2:333237/43‑1 (MQ=255)
                                                                                                                        cGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                                                             >  1:333237/1‑43 (MQ=255)
                                                                                                                                    tGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAAc                                 >  2:280710/1‑107 (MQ=255)
                                                                                                                                    tGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAAc                                 <  1:280710/107‑1 (MQ=255)
                                                                                                                                    tGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAg  >  1:390761/1‑138 (MQ=255)
                                                                                                                                        |                                                                                                                                     
GCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAG  >  NZ_CP009273/3246565‑3246834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑