Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	232,843	T→C	I97T (ATC→ACC)	dnaQ →	DNA polymerase III subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	232,843	0	T	C	100.0%	106.0 / NA	28	I97T (ATC→ACC)	dnaQ	DNA polymerase III subunit epsilon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/11);  total (17/11)

CCCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGG  >  NZ_CP009273/232713‑232972                                                                                                                                   |                                                                                                                                  cccGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACg                                                                                                                           <  2:426629/139‑1 (MQ=255)     aTCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGc                                                                                                                       >  1:388775/1‑139 (MQ=255)            ggtggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGa                                                                                                                 >  2:542067/1‑138 (MQ=255)              tggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGatat                                                                                                              <  1:738461/139‑1 (MQ=255)                      gAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTa                                                                                                      <  2:42736/139‑1 (MQ=255)                      gAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTa                                                                                                      <  1:542067/139‑1 (MQ=255)                                     cATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGtttt                                                                                       >  1:645994/1‑139 (MQ=255)                                              gCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCtt                                                                               >  1:186368/1‑138 (MQ=255)                                               ccGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTaa                                                                             <  1:752137/139‑1 (MQ=255)                                                  aTGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcg                                                                          >  1:362191/1‑139 (MQ=255)                                                                gATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGa                                                            <  1:346238/139‑1 (MQ=255)                                                                        cACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACt                                                    <  2:362191/139‑1 (MQ=255)                                                                              tGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCg                                                                                     >  1:34392/1‑100 (MQ=255)                                                                              tGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCg                                                                                     <  2:34392/100‑1 (MQ=255)                                                                                ccGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGt                                              >  2:731910/1‑137 (MQ=255)                                                                                ccGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGt                                              >  1:486299/1‑137 (MQ=255)                                                                                ccGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGt                                              >  1:206789/1‑137 (MQ=255)                                                                                         ccGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGa                                   <  1:731910/139‑1 (MQ=255)                                                                                                           atatTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATTGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAg                 >  1:122919/1‑139 (MQ=255)                                                                                                           atatTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGt                                         <  2:353454/115‑1 (MQ=255)                                                                                                           atatTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGt                                         >  1:353454/1‑115 (MQ=255)                                                                                                           atatTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAg                 >  2:312854/1‑139 (MQ=255)                                                                                                           atatTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAg                 >  2:492846/1‑139 (MQ=255)                                                                                                                  cggcgcggAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATg          >  1:432456/1‑139 (MQ=255)                                                                                                                    gcgcggAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGtt        >  2:210950/1‑139 (MQ=255)                                                                                                                          aGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGTTATTCCGAAGACCATTACTTCCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCTCgg  >  1:326718/1‑139 (MQ=255)                                                                                                                               gTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGa                                   <  1:56684/101‑1 (MQ=255)                                                                                                                               gTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGa                                   >  2:56684/1‑101 (MQ=255)                                                                                                                                   |                                                                                                                                  CCCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGG  >  NZ_CP009273/232713‑232972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑