Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,263,514	T→C	intergenic (+553/+61)	yhaC → / ← rnpB	YhaC family protein/RNase P RNA component class A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,263,514	0	T	C	100.0%	101.9 / NA	31	intergenic (+553/+61)	yhaC/rnpB	YhaC family protein/RNase P RNA component class A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/13);  total (18/13)

TCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGG  >  NZ_CP009273/3263391‑3263645                                                                                                                            |                                                                                                                                    tCGTGGACAGTCATTCATCTCTCTTCCCCTCCAAACGCAAACACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGc                                                                                                                      <  2:27659/139‑1 (MQ=11)  cGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGcc                                                                                                                     >  1:520805/1‑139 (MQ=37)  cGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGCGGACAGCCATTCATCTTTCTGcc                                                                                                                     >  2:458713/1‑139 (MQ=255)    tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCGAcccc                                                                                                                   >  2:140881/1‑139 (MQ=255)           tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCaa                                                                                                              >  1:565125/1‑137 (MQ=37)           tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCaaac                                                                                                            >  2:503876/1‑138 (MQ=255)                     ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAccc                                                                                                  >  2:280016/1‑139 (MQ=37)                                   aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                    <  2:520805/139‑1 (MQ=37)                                            cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                           <  1:214065/139‑1 (MQ=21)                                            cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                           <  2:650499/139‑1 (MQ=21)                                            cccGCCGAAGCGGGTTGTTACGTAAACCAGTTGAAACGGACCGATAACCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCAATCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTaaa                                                                           <  2:6781/139‑1 (MQ=11)                                             ccgccgAAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                                                                          <  2:505908/139‑1 (MQ=25)                                                        ggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt                                                               <  2:565125/139‑1 (MQ=37)                                                                         ggTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGtt                                              <  1:722180/139‑1 (MQ=38)                                                                          gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTc                                             >  2:207884/1‑139 (MQ=255)                                                                          gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTc                                             >  1:623389/1‑139 (MQ=255)                                                                               aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCg                                        >  1:183401/1‑139 (MQ=255)                                                                                   aCCGATAAGCCGCTTTCTTTTGGGTATAGCCTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGa                                    <  2:281624/139‑1 (MQ=255)                                                                                       aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt                                >  2:430893/1‑139 (MQ=255)                                                                                               ctttctttTGGTTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                           <  2:79141/88‑1 (MQ=255)                                                                                               ctttctttTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                           >  1:79141/1‑88 (MQ=18)                                                                                                        gggTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAg                <  1:430893/138‑1 (MQ=255)                                                                                                         ggTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAg                                                     <  1:46732/100‑1 (MQ=37)                                                                                                         ggTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAg                                                     >  2:46732/1‑100 (MQ=37)                                                                                                              aGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGc         >  1:489840/1‑139 (MQ=255)                                                                                                              aGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGc         >  1:756286/1‑139 (MQ=255)                                                                                                                 gtcgtGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCa      <  2:734266/139‑1 (MQ=255)                                                                                                                     tGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTgg  >  2:491221/1‑139 (MQ=255)                                                                                                                     tGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTgg  >  1:32190/1‑139 (MQ=255)                                                                                                                     tGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTgg  >  2:282995/1‑139 (MQ=255)                                                                                                                     tGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTgg  >  1:64805/1‑139 (MQ=255)                                                                                                                            |                                                                                                                                    TCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGG  >  NZ_CP009273/3263391‑3263645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑