| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,381,255 | A→C | V98G (GTG→GGG) | aaeB ← | p‑hydroxybenzoic acid efflux pump subunit AaeB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,381,255 | 0 | A | C | 97.5% | 150.8 / ‑3.3 | 40 | V98G (GTG→GGG) | aaeB | p‑hydroxybenzoic acid efflux pump subunit AaeB |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (20/19); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GGCGTAAGCAATGGTTCCGGCTGAATGGTGATCACAATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCA > NZ_CP009273/3381120‑3381392 | ggcgTAAGCAATGGTTCCGGCTGAATGGTGATCACAATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGCACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCa < 2:380924/139‑1 (MQ=255) cgTAAGCAATGGTTCCGGCTGAATGGTGATCACAATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGa < 2:50517/139‑1 (MQ=255) tAAGCAATGGTTCCGGCTGAATGGTGATCACAATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAat > 2:680343/1‑139 (MQ=255) tAAGCAATGGTTCCGGCTGAATGGTGATCACAATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGAACCCCAGAat > 2:410729/1‑139 (MQ=255) aGCAATGGTTCCGGCTGAATGGTGATCGCAATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAatc < 1:410729/138‑1 (MQ=255) aGCAATGGTTCCGGCTGAATGGTGATCACAATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAatc < 2:47511/138‑1 (MQ=255) aaTGGTGATCACAATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGcgcg > 2:83485/1‑138 (MQ=255) aTCACAATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCg > 2:403949/1‑139 (MQ=255) aGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCa > 1:555233/1‑139 (MQ=255) aGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCa > 1:577915/1‑139 (MQ=255) gcgGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAatc > 1:431195/1‑99 (MQ=255) gcgGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAatc < 2:431195/99‑1 (MQ=255) gcgGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGat > 1:368711/1‑119 (MQ=255) gcgGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGat < 2:368711/119‑1 (MQ=255) ggCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATa < 2:577915/139‑1 (MQ=255) gCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAatc > 1:447043/1‑87 (MQ=255) gCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAatc < 2:447043/87‑1 (MQ=255) ccAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACa < 2:256519/139‑1 (MQ=255) cAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCAc > 2:125209/1‑123 (MQ=255) cAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCAc < 1:125209/123‑1 (MQ=255) cAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAg > 1:275694/1‑139 (MQ=255) tACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGGGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGc > 2:531520/1‑139 (MQ=255) aCGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGcc > 2:198220/1‑139 (MQ=255) ttCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGatgatg > 1:585759/1‑139 (MQ=255) gTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCaaa > 1:727163/1‑137 (MQ=255) gCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACg < 2:555233/139‑1 (MQ=255) tCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAAt < 1:771894/139‑1 (MQ=255) cAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAg < 2:699658/139‑1 (MQ=255) tACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACa > 2:174482/1‑88 (MQ=255) tACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACa < 1:174482/88‑1 (MQ=255) aaaCCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAAt < 1:737870/139‑1 (MQ=255) ggCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGt > 1:418649/1‑139 (MQ=255) ggCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGt < 2:727163/139‑1 (MQ=255) cccAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTc < 1:404981/139‑1 (MQ=255) aGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATaa > 1:298950/1‑75 (MQ=255) aGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATaa < 2:298950/75‑1 (MQ=255) aGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCg > 2:499751/1‑138 (MQ=255) gCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAatgat < 2:450341/45‑1 (MQ=255) gCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAatgat > 1:450341/1‑45 (MQ=255) gCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCg > 2:127574/1‑137 (MQ=255) caccCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCgcag > 2:546274/1‑139 (MQ=255) cacCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCgcagca < 1:499751/139‑1 (MQ=255) | GGCGTAAGCAATGGTTCCGGCTGAATGGTGATCACAATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCA > NZ_CP009273/3381120‑3381392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |