Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,473,413	A→G	M185T (ATG→ACG)	kefB ←	glutathione‑regulated potassium‑efflux system protein KefB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,473,413	0	A	G	100.0%	113.3 / NA	31	M185T (ATG→ACG)	kefB	glutathione‑regulated potassium‑efflux system protein KefB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/14);  total (17/14)

ATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAG  >  NZ_CP009273/3473279‑3473525                                                                                                                                       |                                                                                                                 aTGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                              <  1:286003/139‑1 (MQ=255) aTGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                              <  1:761619/139‑1 (MQ=255)  tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                              >  1:141950/1‑138 (MQ=255)  tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                              >  2:598087/1‑138 (MQ=255)  tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                              >  1:110257/1‑138 (MQ=255)            aaCACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATc                                                                                                    >  2:478489/1‑138 (MQ=255)                                       aaCACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCg                                                                        >  2:350210/1‑139 (MQ=255)                                                cGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACg                                                               <  2:110257/139‑1 (MQ=255)                                                cGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACg                                                               <  2:57382/139‑1 (MQ=255)                                                      ccAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATg                                                                                         >  1:426371/1‑107 (MQ=255)                                                      ccAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATg                                                                                         <  2:426371/107‑1 (MQ=255)                                                      ccAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACt                                                          >  2:230648/1‑138 (MQ=255)                                                          aaGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGc                                                     <  1:598087/139‑1 (MQ=255)                                                            gCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCa                                                   <  1:350210/139‑1 (MQ=255)                                                            gCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCa                                                   <  1:463625/139‑1 (MQ=255)                                                               gCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTg                                                >  2:9201/1‑139 (MQ=255)                                                               gCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTg                                                >  2:22597/1‑139 (MQ=255)                                                                          gAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACt                                     >  2:18607/1‑139 (MQ=255)                                                                                   aCGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATcc                            <  2:141950/139‑1 (MQ=255)                                                                                       aGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGa                         >  2:453540/1‑138 (MQ=255)                                                                                                 gcCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCt                                              >  1:770557/1‑107 (MQ=255)                                                                                                 gcCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCt                                              <  2:770557/107‑1 (MQ=255)                                                                                                 gcCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAAc              >  1:78678/1‑139 (MQ=255)                                                                                                   ccaccaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCa                                 <  2:369719/118‑1 (MQ=255)                                                                                                   ccaccaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCa                                 >  1:369719/1‑118 (MQ=255)                                                                                                   ccaccaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCg            <  1:453540/139‑1 (MQ=255)                                                                                                     accaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCt                                              <  1:430561/103‑1 (MQ=255)                                                                                                     accaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCt                                              >  2:430561/1‑103 (MQ=255)                                                                                                     accaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGa           >  2:327145/1‑138 (MQ=255)                                                                                                     accaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGa           >  1:635602/1‑138 (MQ=255)                                                                                                             gCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAg  <  1:478489/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                 ATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAG  >  NZ_CP009273/3473279‑3473525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑