Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,639,274 T→C V177A (GTC→GCC)  rlmJ → 23S rRNA (adenine(2030)‑N(6))‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,639,2740TC100.0% 159.7 / NA 44V177A (GTC→GCC) rlmJ23S rRNA (adenine(2030)‑N(6))‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/24);  total (20/24)

AGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGG‑CCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGACCGGTATTCGCAAAATTCTGCA  >  NZ_CP009273/3639139‑3639406
                                                                                                                                         |                                                                                                                                    
aaaaaaATCGCCGGGCGCGTGCCAAAAAACCCG‑CGGTTTCCAACAAGTTAAGG‑CCAAACCGGCCGCCGTTTCCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAg                                                                                                                                   <  2:505500/137‑1 (MQ=255)
    aGATGGCCGGGGGCGTGTAAAAAAACCCGGCGTTTCCCAGCAGTTAAAGC‑CCAAA‑CGGCCGCGGTTTCCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCggg                                                                                                                               <  2:199539/139‑1 (MQ=255)
     gATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGTTTAAGG‑CCAAA‑CTGCCGCCGTTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGa                                                                                                                              <  1:627120/139‑1 (MQ=255)
           cgggcgcGTGTCAAAAAGCCCGAGGGTTTCCAGCAGCTAAAGG‑CCAAC‑CTGCCGCCGGTTCCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAg                                                                                                                        <  2:725011/136‑1 (MQ=255)
           cGTGCGCGTGTCAAAAACGCCGACGTTTCCCAGCAGCTAAAGC‑CCAAC‑CTGCCGCCGGTTCCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAg                                                                                                                        <  2:424804/139‑1 (MQ=255)
            cggcgcGTGTCAAAAAACCCGACGTTTTCCAGCAGCTAAAGC‑CCAAA‑CTGCCGCGGTTTTCCCGCCGTGGTTTAACCCTTACCGCCCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGa                                                                                                                       <  2:743530/137‑1 (MQ=255)
             tgcggttgtCAAAAACGCCGGCGTTTCCCAGCAGTTAAAGG‑CCAAA‑CCGCCCCGGGTTCCCCCCCGTGGTTTAATCCTGCCCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGaa                                                                                                                      <  2:140517/133‑1 (MQ=255)
             tGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGG‑CCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAg                                                                                                                        >  1:75563/1‑137 (MQ=255)
                gcgTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGG‑CCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGt                                                                                                                   >  1:392018/1‑139 (MQ=255)
                    gtCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGG‑CCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTAc                                                                                                                >  1:452916/1‑138 (MQ=255)
                    gtCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGG‑CCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTAc                                                                                                                >  2:210912/1‑138 (MQ=255)
                                gACGGTTTCCAGCAGCTAAAGC‑CAAAC‑CTGCCGCCGTTTCCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCa                                                                                                   <  2:369137/139‑1 (MQ=255)
                                             cgcTTAAGGCCCAAC‑CTGCCGCCGTTTCCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACg                                                                                       <  2:209368/138‑1 (MQ=255)
                                              gcTTAAGT‑CAAAA‑CGCCGGCCGTTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTTGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCa                                                                                     <  1:413340/139‑1 (MQ=255)
                                                  aaag‑CCAAACCTCCCGCCGTTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACt                                                                                   <  1:731683/135‑1 (MQ=255)
                                                          aa‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtgtg                                                                                >  2:17675/1‑133 (MQ=255)
                                                          aa‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtgtg                                                                                <  1:17675/133‑1 (MQ=255)
                                                          aa‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATcc                                                                          >  2:490806/1‑139 (MQ=255)
                                                                ccgccgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtgg                                                                     >  1:522266/1‑139 (MQ=255)
                                                                ccgccgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtgg                                                                     >  2:369178/1‑139 (MQ=255)
                                                                                 tGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAtt                                                    <  1:225923/139‑1 (MQ=255)
                                                                                 tGGTTAACTCTTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAtt                                                    <  2:452916/139‑1 (MQ=255)
                                                                                   gTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAtt                                                    >  2:642402/1‑137 (MQ=255)
                                                                                        acccTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGttt                                                                                                        <  2:723373/78‑1 (MQ=255)
                                                                                        aTCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGttt                                                                                                        >  1:723373/1‑80 (MQ=255)
                                                                                         tCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACt                                                                                                 <  2:609742/86‑1 (MQ=255)
                                                                                         tCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACt                                                                                                 >  1:609742/1‑86 (MQ=255)
                                                                                         tCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCAt                                            <  1:131801/139‑1 (MQ=255)
                                                                                         cccTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCAt                                            <  2:656630/138‑1 (MQ=255)
                                                                                             tATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATc                                        >  1:71263/1‑139 (MQ=255)
                                                                                             tATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATc                                        >  1:283592/1‑139 (MQ=255)
                                                                                             tATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATc                                        >  1:451003/1‑139 (MQ=255)
                                                                                              aTCGAGCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGGTTCGCCACTGGAATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAGGCGCATGATc                                        >  2:81867/1‑138 (MQ=255)
                                                                                              aTCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATc                                        <  1:210912/138‑1 (MQ=255)
                                                                                               tCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCa                                      <  1:642402/139‑1 (MQ=255)
                                                                                               ccGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCa                                      <  2:685894/138‑1 (MQ=255)
                                                                                                   cccGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGAt                                  >  1:504351/1‑139 (MQ=255)
                                                                                                   cccGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGAt                                  >  2:717331/1‑139 (MQ=255)
                                                                                                      gccgTATGAAATGAAAACTGACTATCAAGCGGTGGCCAg                                                                                                                                   >  2:708874/1‑39 (MQ=255)
                                                                                                      gccgTATGAAATGAAAACTGACTATCAAGCGGTGGCCAg                                                                                                                                   <  1:708874/39‑1 (MQ=255)
                                                                                                              aaatgaaaACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGa                        <  2:100015/138‑1 (MQ=255)
                                                                                                                 tgaaaACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGACCgg                    >  2:58649/1‑139 (MQ=255)
                                                                                                                                 aGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCa                                             >  1:580806/1‑98 (MQ=255)
                                                                                                                                 aGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCa                                             <  2:580806/98‑1 (MQ=255)
                                                                                                                                    ggtggcCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGACCGGTATTCGCAAAATTCTGCa  >  1:629125/1‑138 (MQ=255)
                                                                                                                                    ggtggcCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGACCGGTATTCGCAAAATTCTGCa  >  1:697114/1‑138 (MQ=255)
                                                                                                                                         |                                                                                                                                    
AGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGG‑CCAAA‑CTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGACCGGTATTCGCAAAATTCTGCA  >  NZ_CP009273/3639139‑3639406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑