Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,829,452 T→G intergenic (‑163/‑130) yicJ ← / → BW25113_RS18990 glycoside‑pentoside‑hexuronide family transporter/tRNA‑Sec

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,829,4520TG100.0% 91.4 / NA 26intergenic (‑163/‑130)yicJ/BW25113_RS18990glycoside‑pentoside‑hexuronide family transporter/tRNA‑Sec
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/12);  total (14/12)

CTGAGACCGATAACGCATTTTGTAAACCATCCCTGTGAGAGCGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGTTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTGGGGGATGTAGAAACTCAAGGAAGTAGCTATAATGCGCCCCGCCTCCATGTAGCAATCGAGGCGCGG  >  NZ_CP009273/3829317‑3829583
                                                                                                                                       |                                                                                                                                   
cTGAGACCGATAACGCATTTTGTAAACCATCCCTGTGAGAGCGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGgtt                                                                                                                                   >  1:302/1‑138 (MQ=255)
cTGAGACCGATAACGCATTTTGTAAACCATCCCTGTGAGAGCGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGgtt                                                                                                                                   >  1:567362/1‑138 (MQ=255)
cTGAGACCGATAACGCATTTTGTAAACCATCCCTGTGAGAGCGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGgtt                                                                                                                                   >  1:689384/1‑138 (MQ=255)
     aCCGATAACGCATTTTGTAAACCATCCCTGTGAGAGCGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATg                                                                                                                             <  1:436981/139‑1 (MQ=255)
     aCCGATAACGCATTTTGTAAACCATCCCTGTGAGAGCGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATg                                                                                                                             <  1:87649/139‑1 (MQ=255)
                  tttGTAAACCATCCCTGTGAGAGCGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCtt                                                                                                                >  2:311934/1‑139 (MQ=255)
                      tAAACCATCCCTGTGAGAGCGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCg                                                                                                             <  1:125981/138‑1 (MQ=255)
                      tAAACCATCCCTGTGAGAGCGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCg                                                                                                             <  1:628100/138‑1 (MQ=255)
                             tCCCTGTGAGAGCGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGataata                                                                                                     >  1:529724/1‑139 (MQ=255)
                               ccTGTGAGAGCGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACg                                                                                                   <  1:578002/139‑1 (MQ=255)
                                   tgAGAGCGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCt                                                                                                 <  2:689384/137‑1 (MQ=255)
                                         cGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTAt                                                                                         >  2:111147/1‑139 (MQ=255)
                                                     tatCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGaaaa                                                                              >  2:735274/1‑138 (MQ=255)
                                                                     tATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTGGGGGATg                                                             >  1:720874/1‑139 (MQ=255)
                                                                       tGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTGGGGGATGTa                                                           <  2:495790/139‑1 (MQ=255)
                                                                                  ttATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTGGGGGATGTAGAAACTCAAg                                                 >  1:22869/1‑138 (MQ=255)
                                                                                    aTGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTGGGGGATGTAGAAACTCAAGGa                                               <  1:735274/138‑1 (MQ=255)
                                                                                     tgtgATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTGGGGGATGTAGAAACTCAAGGAAg                                             >  1:715280/1‑139 (MQ=255)
                                                                                          tCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTtat                                                                                                                                >  1:418153/1‑51 (MQ=255)
                                                                                          tCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTtat                                                                                                                                <  2:418153/51‑1 (MQ=255)
                                                                                             tGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACg                                                                                                   >  2:311926/1‑77 (MQ=255)
                                                                                             tGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACg                                                                                                   <  1:311926/77‑1 (MQ=255)
                                                                                                                           gTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAGAAACTGATCTGGGGGATGTAGAACCTCAAGGAAGTAGCTATAATGCGCCCCGCCTCCATGTAGCAATCGAgg       >  1:373684/1‑139 (MQ=255)
                                                                                                                           gTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTggggg                                                                >  2:276976/1‑82 (MQ=255)
                                                                                                                           gTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTggggg                                                                <  1:276976/82‑1 (MQ=255)
                                                                                                                           gTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTGGGGGATGTAGAAACTCAAGGAAGTAGCTATAATGCGCCCCGCCTCCATGTAGCAATCGAgg       >  2:108003/1‑139 (MQ=255)
                                                                                                                           gTAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTGGGGGATGTAGAAACTCAAGGAAGTAGCTATAATGCGCCCCGCCTCCATGTAGCAATCGAgg       >  1:608430/1‑139 (MQ=255)
                                                                                                                            tAAAATATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTGGGGGATGTAGAAACTCAAGGAAGTAGCTATAATGCGCCCCGCCTCCATGTAGCAATCGAGgc      >  1:669642/1‑139 (MQ=255)
                                                                                                                              aaaTATGGGGTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTGGGGGATGTAGAAACTCAAGGAAGTAGCTATAATGCGCCCCGCCTCCATGTAGCAATCGAGgcg     <  2:567362/138‑1 (MQ=255)
                                                                                                                                    ggggTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTGGGGGATGTAGAAACTCAAGGAAGTAGCTATAATGCGCCCCGCCTCCATGTAGCAATCGAGGCGCgg  >  2:348919/1‑135 (MQ=255)
                                                                                                                                       |                                                                                                                                   
CTGAGACCGATAACGCATTTTGTAAACCATCCCTGTGAGAGCGAATATTCCGATATCTGGTTATTAATTTATGATTCTTGTTTTATGTGATCGTGGTAGCGTTAATTCCGCTCATATATCATTGTAAAATATGGGTTTTATATGAACTATAATGCTTTCGTGATAATACGCTGCGTGTATTAGGCGGAAAAAACTGATCTGGGGGATGTAGAAACTCAAGGAAGTAGCTATAATGCGCCCCGCCTCCATGTAGCAATCGAGGCGCGG  >  NZ_CP009273/3829317‑3829583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑