Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,876,284	T→C	D269G (GAT→GGT)	dnaA ←	chromosomal replication initiator protein DnaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,876,284	0	T	C	100.0%	195.2 / NA	54	D269G (GAT→GGT)	dnaA	chromosomal replication initiator protein DnaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/31);  total (23/31)

TCGTTTTCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGATCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGGTAGTAGC  >  NZ_CP009273/3876149‑3876419                                                                                                                                        |                                                                                                                                        tCGTTTTCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGAccc                                                                                                                                       >  2:173200/1‑138 (MQ=255)                      atcaGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGAt                                                                                                                 <  1:439978/139‑1 (MQ=255)                                ccACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcag                                                                                                       >  1:520510/1‑139 (MQ=255)                                   ctcGGGTTTCCAGTTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagcag                                                                                                    <  2:433441/137‑1 (MQ=255)                                   cacGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagcag                                                                                                    <  1:173200/139‑1 (MQ=255)                                   cacGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagcag                                                                                                    <  2:434409/139‑1 (MQ=255)                                    angtgTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGAt                                                                                                                 <  2:164106/121‑1 (MQ=255)                                     cGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGAt                                                                                                                 >  2:755995/1‑124 (MQ=255)                                     cGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGAt                                                                                                                 <  1:755995/124‑1 (MQ=255)                                           tCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTg                                                                                             <  1:243924/138‑1 (MQ=255)                                           tCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTg                                                                                             >  1:423026/1‑138 (MQ=255)                                                 tctGGCGGTTCGATCGCCACCGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATc                                                                                                                        >  2:359199/1‑105 (MQ=255)                                                 tctGGCGGTGCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATc                                                                                                                        <  1:359199/105‑1 (MQ=255)                                                  ctGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGAt                                                                                                                         >  2:244631/1‑103 (MQ=255)                                                  ctGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGAt                                                                                                                         <  1:244631/103‑1 (MQ=255)                                                       ggTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGAt                                                                                                                 <  2:499789/106‑1 (MQ=255)                                                       ggTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGAt                                                                                                                 >  1:499789/1‑106 (MQ=255)                                                           cGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAAct                                                                             <  2:485476/138‑1 (MQ=255)                                                           cGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAAct                                                                             <  1:34137/138‑1 (MQ=255)                                                                gCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTcc                                                                        <  2:386768/138‑1 (MQ=255)                                                                          gtcCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTc                                                             >  1:440786/1‑139 (MQ=255)                                                                           tcCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCt                                                            >  1:291205/1‑139 (MQ=255)                                                                               cAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTttat                                                        >  2:106371/1‑139 (MQ=255)                                                                               cAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTttat                                                        >  2:178269/1‑139 (MQ=255)                                                                                 aCCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCg                                                                                                                                      <  2:531741/59‑1 (MQ=255)                                                                                 aCCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCg                                                                                                                                      >  1:531741/1‑59 (MQ=255)                                                                                 aCCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGAt                                                                                                                         >  1:461686/1‑72 (MQ=255)                                                                                 aCCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGAt                                                                                                                         <  2:461686/72‑1 (MQ=255)                                                                                     aaGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCa                                                   >  1:321136/1‑138 (MQ=255)                                                                                      aGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCaaa                                                 <  1:267660/139‑1 (MQ=255)                                                                                         gggATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCaaaaaa                                              <  1:81620/139‑1 (MQ=255)                                                                                             tttCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGAt                                                                  >  1:527558/1‑115 (MQ=255)                                                                                             tttCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGAt                                                                  <  2:527558/115‑1 (MQ=255)                                                                                                   aCGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATAtcg                                    <  2:321136/139‑1 (MQ=255)                                                                                                      aTCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATAtcgtcg                                 <  2:15268/139‑1 (MQ=255)                                                                                                            aaCGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATcagc                           <  2:586411/139‑1 (MQ=255)                                                                                                            aaCGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACCGAATATCGTCGATcag                            <  1:703171/138‑1 (MQ=255)                                                                                                                 cGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTg                       >  2:525454/1‑138 (MQ=255)                                                                                                                 cGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTg                       >  1:339928/1‑138 (MQ=255)                                                                                                                 cGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTg                       >  2:738877/1‑138 (MQ=255)                                                                                                                   ttGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAAct                                                                             >  1:465460/1‑82 (MQ=255)                                                                                                                   ttGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAAct                                                                             <  2:465460/82‑1 (MQ=255)                                                                                                                   ttGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCaaa                                                 <  2:404588/110‑1 (MQ=255)                                                                                                                   ttGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCaaa                                                 >  1:404588/1‑110 (MQ=255)                                                                                                                   ttGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAA‑GTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATc                   >  2:416387/1‑139 (MQ=255)                                                                                                                   ttGATCTCTTTCGGATAGCGACCCGAGGCGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAAATGAATATCGTCGATCAGCAGTGCAt                    >  1:579093/1‑139 (MQ=255)                                                                                                                           tttCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACgg              <  1:338154/137‑1 (MQ=255)                                                                                                                             tCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGaa            <  2:95651/137‑1 (MQ=255)                                                                                                                             tCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGaa            <  1:738877/137‑1 (MQ=255)                                                                                                                             tCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGaa            <  1:52812/137‑1 (MQ=255)                                                                                                                             tCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGaa            <  1:275673/137‑1 (MQ=255)                                                                                                                             tCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACg          >  2:252916/1‑139 (MQ=255)                                                                                                                               ggATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACgg         <  2:110233/138‑1 (MQ=255)                                                                                                                                  tAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGgtagt     <  1:380740/139‑1 (MQ=255)                                                                                                                                     cGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAACAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGGTAGTAGc  >  1:189405/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        TCGTTTTCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGATCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGGTAGTAGC  >  NZ_CP009273/3876149‑3876419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑