Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,937,289	A→G	noncoding (226/2904 nt)	BW25113_RS19530 →	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,937,289	0	A	G	100.0%	43.9 / NA	22	noncoding (226/2904 nt)	BW25113_RS19530	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/8);  total (14/8)

ACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCA  >  NZ_CP009273/3937219‑3937415                                                                       |                                                                                                                               aCTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt                                                              >  2:569880/1‑137 (MQ=17) aCTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt                                                              >  2:406841/1‑137 (MQ=17)   tGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGt                                                          >  1:561019/1‑139 (MQ=17)     aaTCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTg                                                         >  2:484339/1‑138 (MQ=17)       tCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTgg                                                        >  2:216234/1‑137 (MQ=17)          aTAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCg                                                   >  2:122880/1‑139 (MQ=17)           tAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACAACGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGt                                                  >  1:368781/1‑139 (MQ=12)            aGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTc                                                 >  2:660643/1‑139 (MQ=17)                 aaTGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGc                                                    <  2:390452/131‑1 (MQ=17)                 aaTGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGc                                                    >  1:390452/1‑131 (MQ=17)                        cGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt                                                                  <  2:384870/110‑1 (MQ=17)                        cGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt                                                                  >  1:384870/1‑110 (MQ=17)                        cGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGgcgcg                                     <  1:569880/139‑1 (MQ=17)                                     cTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGa                        <  1:122880/139‑1 (MQ=17)                                                 aGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTaca            <  2:242935/139‑1 (MQ=17)                                                     ccccGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACaaa        <  1:484339/139‑1 (MQ=17)                                                       ccGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACaaaaa      <  2:640839/139‑1 (MQ=17)                                                          aGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATg    >  1:29322/1‑138 (MQ=17)                                                           ggAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt                                                                    <  1:479802/73‑1 (MQ=17)                                                           ggAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt                                                                    >  2:479802/1‑73 (MQ=17)                                                           ggAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGca  >  1:63034/1‑139 (MQ=17)                                                           ggAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGca  >  1:411031/1‑139 (MQ=17)                                                                       |                                                                                                                               ACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCA  >  NZ_CP009273/3937219‑3937415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑